Convergent but temporally separated inputs to lateral amygdala neurons from the auditory thalamus and auditory cortex use different postsynaptic receptors: In vivo intracellular and extracellular recordings in fear conditioning pathways
Convergent but temporally separated inputs to lateral amygdala neurons from the auditory thalamus and auditory cortex use different postsynaptic receptors: In vivo intracellular and extracellular recordings in fear conditioning pathways
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DOI：
10.1101/lm.3.2-3.229
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发表时间：
1996-09-01
影响因子：
2
通讯作者：
LeDoux, JE
中科院分区：
文献类型：
--
作者：
Li, XF;Stutzmann, GE;LeDoux, JE
杏仁核（LA）的外侧核是恐惧调节回路的关键组成部分，它从听觉丘脑接收快速但相对贫乏的听觉输入，从听觉皮层接收较慢但较丰富的输入。我们在氨基甲酸乙酯麻醉的大鼠中检查了 LA 中的单个细胞是否接收来自这两个区域的汇聚输入，以及不同的突触后受体是否有助于两条通路上的时间分离的兴奋。通过细胞外和细胞内的记录，单个细胞可以通过刺激每个通路而被激活。在细胞外记录中，离子导入应用 N-甲基-D-天冬氨酸 (NMDA) 受体拮抗剂 APV 和 L-α-氨基-3-羟基-5-甲基-4-异恶唑丙酸酯 (AMPA) 受体拮抗剂 CNQX 证明，两种途径中的突触传递依赖于 AMPA 受体，而丘脑途径中的传递也依赖于 NMDA 受体的参与。细胞内记录证实 NMDA 受体参与丘脑而非皮质 LA 的突触激活。
The lateral nucleus of the amygdala (LA), a key component of the fear conditioning circuitry, receives a rapid but relatively impoverished auditory input from the auditory thalamus and a slower but richer input from the auditory cortex. We examined in urethane anesthetized rats whether individual cells in the LA receive convergent inputs from these two areas, and whether different postsynaptic receptors contribute to the temporally separated excitations over the two pathways. With both extracellular and intracellular recordings, individual cells could be activated by stimulation of each pathway. in extracellular recordings iontophoretic application of the N-methyl-D-aspartate (NMDA) receptor antagonist APV and the: L-alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor antagonist CNQX demonstrated that synaptic transmission in both pathways depends on AMPA receptors, whereas transmission in the thalamic pathway also depends on the involvement of NMDA receptors. The involvement of NMDA receptors in synaptic activation of the LA from the thalamus but not the cortex was confirmed in intracellular recordings.