The distribution and cellular localization of CRF-R1 in the vermis of the postnatal mouse cerebellum
The distribution and cellular localization of CRF-R1 in the vermis of the postnatal mouse cerebellum
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DOI：
10.1006/exnr.2002.8052
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发表时间：
2002-12-01
影响因子：
5.3
通讯作者：
Bishop, GA
中科院分区：
文献类型：
--
作者：
King, JS;Bishop, GA
促肾上腺皮质激素释放因子（CRF）的分布，CRF结合位点的发展，和年龄时，应用CRF elerably生理反应已在出生后的小鼠小脑中描述。本研究的目的是确定出生后小鼠小脑蚓部中1型CRF受体（CRF-R1）的细胞和亚细胞分布，并将这些数据与之前研究中提供的数据相关联。在P0，CRF-R1存在于迁移性浦肯野细胞的顶端过程中。在P0和P8之间，CRF-R1免疫染色仅限于浦肯野细胞体的核上位置。在P9和P14之间，受体免疫标记包围浦肯野细胞核并延伸到它们的初级树突。P16和P21之间实现了类似成人的分布。在P0和P14之间，CRF-R1抗体还标记了直接朝向软膜表面的迁移GABA能中间神经元的过程。到P12，标记开始限制在内部颗粒细胞层中的GABA能细胞的核内。最后，星形胶质细胞过程中的白色的问题，以及放射状胶质细胞的过程，显示局灶性标记与CRF-R1抗体开始在P3和整个出生后的发展。先前的研究表明，CRF不会引起浦肯野细胞的生理反应，直到P9。这一观察结果，连同在这项研究中提出的数据，表明CRF的1型受体的结合可能参与调节小脑神经元和神经胶质细胞的发育出生后立即，CRF承担其功能之前，作为一种神经调节剂在出生后的发展和成人。(C)2002 Elsevier Science（美国）。
The distribution of corticotropin-releasing factor (CRF), the development of CRF-binding sites, and the age at which application of CRF elicits a physiological response have been described previously in the postnatal mouse cerebellum. The intent of the present study was to determine the cellular and subcellular distribution of the CRF type 1 receptor (CRF-R1) in the vermis of the postnatal mouse cerebellum and to correlate these data with those presented in previous studies. On P0, CRF-R1 is present in the apical processes of migrating Purkinje cells. Between P0 and P8, CRF-R1 immunostaining is confined to a supranuclear position in Purkinje cell bodies. Between P9 and P14, the receptor immunolabeling circumscribes Purkinje cell nuclei and extends into their primary dendrites. An adult-like distribution is achieved between P16 and P21. Between P0 and P14, the CRF-R1 antibody also labels processes of migrating GABAergic interneurons that are directed toward the pial surface. By P12, labeling begins to circumscribe the nucleus of GABAergic cells in the internal granule cell layer. Finally, astrocytic processes in the white matter, as well as radial glial processes, show focal labeling with the CRF-R1 antibody beginning at P3 and throughout postnatal development. A previous study demonstrated that CRF does not elicit a physiological response in Purkinje cells until P9. This observation, together with the data presented in this study, suggests that the binding of CRF to the type 1 receptor may be involved in regulating the development of cerebellar neurons and glia immediately after birth, before CRF assumes its function as a neuromodulator later in postnatal development and in the adult. (C) 2002 Elsevier Science (USA).