Combination of lycopene, quercetin and poloxamer 188 alleviates anxiety and depression in 3-nitropropionic acid-induced Huntington's disease in rats.
Combination of lycopene, quercetin and poloxamer 188 alleviates anxiety and depression in 3-nitropropionic acid-induced Huntington's disease in rats.
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DOI：
10.5455/jice.20140903012921
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
Gangshettiwar A
中科院分区：
文献类型：
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作者：
Jain D;Gangshettiwar A
本研究探讨番茄红素、槲皮素和poloxamer188联合治疗3-硝基丙酸（3-NP）诱导的亨廷顿病（HD）的协同效应。3-NP （10 mg/kg）腹腔灌胃14 d，诱导雄性Wistar大鼠焦虑和抑郁。在第1、7和14天评估体重，在实验结束时评估运动、焦虑和抑郁。3-NP可引起HD样症状，第7天和第14天体重显著下降（P < 0.01），光照区运动时间和进入次数进一步减少，静止时间增加。单独给予番茄红素和槲皮素处理的大鼠体重和运动计数明显恢复，焦虑和抑郁症状明显减轻。然而，番茄红素和槲皮素联合与不加波洛沙姆188治疗对亨廷顿对照大鼠的体重影响更为显著，但两组间无显著影响。然而，与单一药物治疗组相比，联合治疗组在运动、光区停留时间和进入次数方面显著增加，并且静止时间缩短。番茄红素和槲皮素联合或不联合波洛沙姆188治疗HD患者比单一药物治疗更能有效缓解焦虑和抑郁。
The present study investigates synergistic effect of combination treatment of lycopene, quercetin and poloxamer188 in 3-nitropropionic acid (3-NP)-induced Huntington’s disease (HD). Anxiety and depression were induced in male Wistar rat by intra-peritoneal administration of 3-NP (10 mg/kg) for 14 days. Body weight was assessed on day 1, 7 and 14, whereas locomotion, anxiety and depression were assessed at the end of the experiment. Administration of 3-NP induces HD like symptoms and produced a significant decrease in body weight on day 7 (P < 0.01)and day 14 (P < 0.001), further decreased locomotion, time spent and number of entries in light area as well as increased immobility period were observed. The rats treated with lycopene and quercetin alone significantly restore the body weight and locomotion count as well as alleviate anxiety and depression. However, combination treatment of lycopene and quercetin with and without poloxamer 188 produced more significant effect on body weight compared to Huntington control rats, but no significant effect was found between the treated groups. However, significant increased on locomotion, time spent and number of entries in light area and decreased immobility period was observed in the combination treated groups when compared to single drug therapy. Combination treatment of lycopene and quercetin with and without poloxamer 188 in HD more effectively alleviate anxiety and depression than single drug therapy.