Measurement of Tryptophan in Peptides by Acid Hydrolysis in the Presence of Phenol and its Application to the Amino Acid Sequence of a Sea Anemone Toxin
Measurement of Tryptophan in Peptides by Acid Hydrolysis in the Presence of Phenol and its Application to the Amino Acid Sequence of a Sea Anemone Toxin
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苯酚存在下酸水解测定肽中色氨酸及其在海葵毒素氨基酸序列中的应用
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
H. Kamiya
中科院分区：
文献类型：
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作者：
K. Muramoto;S. Sunahara;H. Kamiya
将苯酚(约1%)添加到6M HCl中，在110℃下22小时的多肽水解过程中，很大程度上防止了色氨酸的破坏。色氨酸的回收率取决于6M含酚盐酸的体积和苯酚的浓度。对于标准氨基酸混合物，色氨酸的最大回收率为85%。蛋白质的回收率略低。这种水解法有利于采用柱前标记技术的常规高效液相色谱分析微量氨基酸。将该方法用于青海花菇海葵毒素的次要成分的氨基酸序列分析。该毒素由48个氨基酸残基和3个色氨酸残基组成。
The addition of phenol (about 1%) to 6 m HCl largely prevented destruction of tryptophan during hydrolysis of peptides at 110°C for 22 hr. Tryptophan recovery depended on the volume of 6 m HCl containing phenol and the concentration of phenol. The maximum tryptophan recovery was 85% for a standard amino acid mixture. The recovery was slightly lower for proteins. This hydrolytic procedure was advantageous for micro amino acid analysis using a conventional high-performance liquid chromatography with a precolumn labeling technique. The method was used in the amino acid sequence analysis of a minor component of sea anemone toxins isolated from Anthopleura fuscoviridis. The toxin consisted of 48 amino acid residues with three tryptophan residues.