Synthesis and monitored selection of nucleotide surrogates for binding T:A base pairs in homopurine-homopyrimidine DNA triple helices.
Synthesis and monitored selection of nucleotide surrogates for binding T:A base pairs in homopurine-homopyrimidine DNA triple helices.
复制标题
用于结合同型嘌呤-同型嘧啶 DNA 三螺旋中的 T:A 碱基对的核苷酸替代物的合成和监测选择。
DOI：
10.1093/nar/29.17.3674
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发表时间：
2001
影响因子：
14.9
通讯作者：
Richert,C
中科院分区：
文献类型：
--
作者：
Mokhir,AA;Connors,WH;Richert,C
通过固相合成法共制备了16个序列为5′-TCTTCTZTCTTTCT-3′的寡脱氧核苷酸，其中Z表示N-酰基-N-（2-羟乙基）甘氨酸残基。测试了这些寡核苷酸与双链体5′-AGAAGATAGAAAGA-HEG-TCTTTCTATCTTCT-3′形成三链体的能力，其中HEG是六乙二醇接头。在这些三链体中，一个“中断”T：A碱基对面对第三链中的Z残基。在Ztested的酰基部分中，以甘氨酸连接的蒽醌羧酸残基形成的三链体最稳定，其紫外熔点比以5′-TCTTCTGTCTTTCT-3′为第三链的三链体高8.4°C。从探索性核酸酶选择实验的结果表明，能够识别混合序列的三螺旋形成链的组合搜索是可行的。
A total of 16 oligodeoxyribonucleotides of general sequence 5′-TCTTCTZTCTTTCT-3′, whereZdenotes anN-acyl-N-(2-hydroxyethyl)glycine residue, were prepared via solid phase synthesis. The ability of these oligonucleotides to form triplexes with the duplex 5′-AGAAGATAGAAAGA-HEG-TCTTTCTATCTTCT-3′, where HEG is a hexaethylene glycol linker, was tested. In these triplexes, an ‘interrupting’ T:A base pair faces theZresidue in the third strand. Among the acyl moieties ofZtested, an anthraquinone carboxylic acid residue linked via a glycinyl group gave the most stable triplex, whose UV melting point was 8.4°C higher than that of the triplex with 5′-TCTTCTGTCTTTCT-3′ as the third strand. The results from exploratory nuclease selection experiments suggest that a combinatorial search for strands capable of recognizing mixed sequences by triple helix formation is feasible.