A particle agglutination assay for rapid identification of heparin binding to coagulase-negative staphylococci
A particle agglutination assay for rapid identification of heparin binding to coagulase-negative staphylococci
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DOI：
10.1099/00222615-45-4-263
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发表时间：
1996-10-01
影响因子：
3
通讯作者：
Wadstrom, T
中科院分区：
文献类型：
--
作者：
Pascu, C;Hirmo, S;Wadstrom, T
通过颗粒凝集实验研究了6种不同种类的凝固酶阴性葡萄球菌的肝素结合特性，将经高碘酸钠温和处理的肝素（mol.wt 4000-6000）共价偶联到氨基修饰的乳胶珠（直径0.72 μ m）上，通过将结果与凝固酶阴性葡萄球菌菌株在肝素化微滴定板上的粘附（粘附细胞的百分比）进行比较，验证了颗粒凝集实验的有效性。在38株不同的凝固酶阴性葡萄球菌中，30株对肝素包被乳珠表现出凝集反应性，不同凝固酶阴性葡萄球菌对肝素包被乳珠的凝集反应程度不同（如溶血葡萄球菌的细胞比表皮葡萄球菌的细胞反应更强烈），凝集反应被褐藻聚糖、苏拉明、λ -卡拉胶等硫酸盐化合物显著抑制。葡萄球菌细胞与肝素包被的乳胶珠的凝集被细胞表面提取物完全阻断，而非硫酸盐碳水化合物聚合物如透明质酸则不能。这些结果表明，负责肝素结合的结构暴露在细胞表面。
The heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay, Heparin (mol.wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 mu m diameter), The particle agglutination assay was validated by comparing results with the adhesion (percentage binding of adherent cells) of coagulase-negative staphylococcal strains to heparinised microtitration plates, Of 38 different coagulase-negative staphylococcal strains tested, 30 showed agglutination reactivity with heparin-coated latex beads, Strains of different coagulase-negative staphylococcal species agglutinated heparin-coated latex beads to various extents (e.g., cells of Staphylococcus haemolyticus strains reacted more strongly than cells of S. epidermidis strains), The agglutination reaction was significantly inhibited by fucoidan, suramin, lambda-carrageenan and other sulphated compounds, but not by non-sulphated carbohydrate polymers such as hyaluronic acid, Agglutination of staphylococcal cells with heparin-coated latex beads was completely blocked by a cell-surface extract. These results suggest that structures responsible for heparin binding are exposed on the cell surface.