Differential expression of pleiotrophin and midkine in advanced neuroblastomas.
Differential expression of pleiotrophin and midkine in advanced neuroblastomas.
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发表时间：
1995-04
期刊：
影响因子：
11.2
通讯作者：
Akira Nakagawara;Jeffrey Milbrandt;Takashi Muramatsu;Thomas F. Deuel;Huaqing Zhao;Avital Cnaan
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文献类型：
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作者：
Akira Nakagawara;Jeffrey Milbrandt;Takashi Muramatsu;Thomas F. Deuel;Huaqing Zhao;Avital Cnaan
多效营养因子（PTN）和中期因子（MK）是一个新的神经营养因子家族的成员，其表达受发育调节。PTN也转化NIH 3 T3细胞，MK对某些细胞系具有促有丝分裂作用。神经母细胞瘤是来源于神经嵴细胞的肿瘤，最近的研究表明，这些肿瘤的生物学至少部分受神经营养因子及其受体的调节。为了检测PTN和MK在神经母细胞瘤中的表达，我们分析了它们在72个原发性神经母细胞瘤和11个神经母细胞瘤细胞系以及其他组织和细胞系中的mRNA表达。PTN在良好的神经母细胞瘤中高度表达（I、II和IV-S期，n = 44），而在晚期肿瘤中以显著较低的水平表达（III和IV期，n = 28，P = 0.003）。PTN在具有N-myc扩增的侵袭性神经母细胞瘤或神经母细胞瘤细胞系中均不表达。PTN与神经生长因子高亲和力受体TRK-A的表达模式相似，PTN的表达与良好的预后相关（P < 0.004）。相反，MK在几乎所有的原发性神经母细胞瘤和细胞系中高度表达，并且与疾病分期或N-myc扩增无关。提示PTN和MK的差异表达可能在神经母细胞瘤的生长和分化中起重要作用。
Pleiotrophin (PTN) and midkine (MK) are members of a new family of neurotrophic factors whose expression is developmentally regulated. PTN also transforms NIH 3T3 cells, and MK is mitogenic to certain cell lines. Neuroblastomas are tumors derived from neural crest cells, and recent studies have revealed that the biology of these tumors is at least partly regulated by neurotrophic factors and their receptors. To examine the expression of PTN and MK in neuroblastoma, we analyzed their mRNA expression in 72 primary neuroblastomas and 11 neuroblastoma cell lines as well as other tissues and cell lines. PTN is highly expressed in favorable neuroblastomas (stages I, II, and IV-S, n = 44), whereas it is expressed at a significantly lower level in advanced tumors (stages III and IV, n = 28, P = 0.003). PTN is not expressed in either aggressive neuroblastomas with N-myc amplification or in neuroblastoma cell lines. Moreover, the expression pattern of PTN was similar to that of TRK-A, the high affinity receptor for nerve growth factor, in that it is correlated with a favorable prognosis (P < 0.004). In contrast, MK is highly expressed in almost all primary neuroblastomas and cell lines and showed no correlation with disease stage or N-myc amplification. These results suggest that differential expression of PTN and MK may have an important role in regulating growth and differentiation of neuroblastomas.