CD56: a useful tool for the diagnosis of small cell lung carcinomas on biopsies with extensive crush artefact
CD56: a useful tool for the diagnosis of small cell lung carcinomas on biopsies with extensive crush artefact
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DOI：
10.1136/jcp.2004.023044
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发表时间：
2005-09-01
影响因子：
3.4
通讯作者：
Sheppard, MN
中科院分区：
文献类型：
--
作者：
Kontogianni, K;Nicholson, AG;Sheppard, MN
背景/目的：支气管活检对小细胞肺癌（SCLC）的诊断常因强烈的挤压伪影而存在问题。现在有几种抗体可以帮助诊断小细胞肺癌，并在这种临床情况下评估其价值。方法/结果：20例小细胞肺癌和10例对照组（一个非霍奇金淋巴瘤，三个非小细胞癌，一个滤泡反应性增生，和五个慢性非特异性炎症）与广泛的压碎伪影使用抗体染色CD 56，MNF 116，甲状腺转录因子1（TTF-1）和CD 45。所有SCLC在75 - 100%的可识别肿瘤细胞中均显示出CD 56强阳性染色，即使在存在广泛挤压伪影的区域也是如此。20例中有18例TTF-1阳性，20例中有16例肿瘤细胞中有MNF 116阳性，但这两种抗体在压碎伪影区域几乎没有染色。包括淋巴细胞的对照情况下，CD 45在压碎artefact.Conclusion领域呈阳性，但所有的情况下，小细胞肺癌是negative：CD 56，沿着与标记细胞角蛋白- TTF-1，和CD 45-是有用的诊断小细胞肺癌活检广泛压碎artefact，可以帮助确认诊断的情况下，功能是模棱两可的。
Background/Aims: The diagnosis of small cell lung carcinoma (SCLC) on bronchial biopsy is often problematical as a result of intense crush artefact. Several antibodies are now available to help in the diagnosis of SCLC and their value was assessed in this clinical situation.Methods/Results: Twenty cases of SCLC and 10 control cases ( one non-Hodgkin lymphoma, three non-small cell carcinomas, one follicular reactive hyperplasia, and five chronic non-specific inflammations) with extensive crush artefact were stained using antibodies to CD56, MNF116, thyroid transcription factor 1 (TTF-1), and CD45. All SCLCs showed strong positive staining for CD56 in 75 - 100% of recognisable tumour cells, even in areas where there was extensive crush artefact. Eighteen of 20 cases were positive for TTF-1 and 16 of 20 were positive for MNF116 in the tumour cells, but both of these antibodies showed little or no staining in areas of crush artefact. Control cases comprising lymphoid cells were positive for CD45 in areas of crush artefact, but all cases of SCLC were negative.Conclusion: CD56, along with markers for cytokeratins - TTF-1, and CD45 - are useful in the diagnosis of SCLC in biopsies with extensive crush artefact and can help confirm the diagnosis in cases where features are equivocal.