Predominance of CK2α over CK2α' in the mammalian brain
Predominance of CK2α over CK2α' in the mammalian brain
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DOI：
10.1007/s11010-011-0963-6
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发表时间：
2011-10-01
影响因子：
4.3
通讯作者：
Rebholz, Heike
中科院分区：
文献类型：
--
作者：
Ceglia, Ilaria;Flajolet, Marc;Rebholz, Heike
CK2是一种由两个催化亚基和两个调节亚基组成的异四聚体普遍存在的蛋白激酶。两个催化亚基α和α‘高度同源，但在C末端区域不同。目前尚不清楚CK2α和α‘是否具有不同的底物特异性，因为还没有鉴定出α或α’特异的底物。因此，假设这两种激酶亚型在底物专一性上是重叠的。研究发现，与其他器官相比，大脑中CK2的蛋白水平和活性都有所提高。在这里，我们研究了CK2α和α‘亚型在九个主要脑区的蛋白水平。我们发现，CK2α和α‘这两个基因在所有被测试的大脑区域都有表达。虽然CK2α水平在不同区域之间变化不大，但大脑皮质和海马区的CK2α水平略高于其他区域。此外，我们还发现，与CK2α相比，纹状体中CK2α的蛋白水平相对较高。CK2α：CK2α‘的化学计量比约为8：1。因此，可以认为CK2α水平在哺乳动物大脑中占主导地位。
CK2 is a heterotetrameric ubiquitous kinase consisting of two catalytic subunits and two regulatory subunits. The two catalytic subunits, alpha and alpha', are highly homologous but differ in their C-terminal regions. It is not known whether CK2 alpha and alpha' have distinctive substrate specificity, since no alpha- or alpha'-specific substrate has been identified. Thus, it is assumed that the two kinase isoforms overlap in their substrate specificity. CK2 protein levels and activity were found to be elevated in the brain when compared to other organs. Here we have studied the protein levels of CK2 alpha and alpha' isoforms in nine major brain regions. We found that both, CK2 alpha and alpha', are expressed in all brain regions tested. Whereas CK2 alpha levels do not vary strongly across the regions, CK2 alpha' levels are slightly higher in the cortex and hippocampus than in other regions. Furthermore, we show that CK2 alpha protein levels in the striatum are relatively high when compared to CK2 alpha'. The approximate stoichiometry ratio of CK2 alpha:CK2 alpha' is 8:1. Therefore, one can consider that CK2 alpha levels are predominant in comparison to CK2 alpha' levels throughout the mammalian brain.