The identification of lymphoid cell subpopulations in sections of human lymphoid tissue and gingivitis in children using monoclonal antibodies.
The identification of lymphoid cell subpopulations in sections of human lymphoid tissue and gingivitis in children using monoclonal antibodies.
复制标题
使用单克隆抗体鉴定人体淋巴组织切片中的淋巴细胞亚群和儿童牙龈炎。
DOI：
10.1111/j.1600-0765.1982.tb01151.x
复制
发表时间：
1982
影响因子：
3.5
通讯作者：
G. Burns
中科院分区：
文献类型：
--
作者：
G. Seymour;M. S. Crouch;R. N. Powell;D. Brooks;I. Beckman;H. Zola;J. Bradley;G. Burns
针对淋巴细胞分化抗原的单克隆抗体用于表征人类淋巴组织和与儿童乳牙列相关的发炎牙龈组织切片中淋巴细胞亚群的表型。使用四种单克隆抗体，命名为FMC 1、FMC 4、FMC 7和UCHT 1。这些抗体分别对B-细胞、p28，33（la-样）抗原、B-细胞亚群和外周T-细胞具有特异性。FMC 1和FMC 4阳性细胞（B-细胞）主要见于次级卵泡，而UCHT 1阳性细胞（T-细胞）见于人扁桃体的滤泡旁区域。扁桃体中部分（但不是全部）次级滤泡的细胞呈FMC 7阳性。在牙龈组织中，仅12.6%的浸润细胞为FMC 1阳性，12.2%为FMC 4阳性，4.8%为FMC 7阳性。另一方面，超过75%的细胞似乎是UCHT 1阳性的。这些结果表明，与儿童乳牙列相关的牙龈炎中的大多数炎性细胞具有表型B-细胞抗原-/p28，33（lalike）抗原-/B-细胞亚群抗原-和可能的T-细胞抗原+。这样的表型，至少通过排除，提示T细胞，因此证实了使用T细胞酶标记物的早期研究。版权
Monoclonal antibodies made against lymphocyte differentiation antigens were used to characterize phenotypically the lymphoid cell subpopulations in sections of human lymphoid tissue and inflamed gingival tissue associated with the deciduous dentition in children. Four monoclonal antibodies designated FMC 1, FMC 4, FMC 7, and UCHT1 were used. These antibodies are specific for B‐cells, p28,33 (la‐like) antigen, a B‐cell subset, and peripheral T‐cells respectively. FMC 1 and FMC 4 positive cells (B‐cells) were found mainly in the secondary follicles while UCHT1 positive cells (T‐cells) were found in the parafollicular areas of human tonsils. Cells in some, but not all, secondary follicles in the tonsils were FMC 7 positive. In the gingival tissue only 12.6 % of the infiltrating cells were FMC 1 positive, 12.2 % were FMC 4 positive, and 4.8 % FMC 7 positive. On the other hand over 75 % of cells appeared to be UCHT1 positive. These results indicate that the majority of inflammatory cells in gingivitis associated with the deciduous dentition in children have the phenotype B‐cell antigen‐/p28,33 (lalike) antigen‐/B‐cell subset antigen‐ and possibly T‐cell antigen+. Such a phenotype is, at least by exclusion, suggestive of T‐cells and as such confirms earlier studies using T‐cell enzyme markers. Copyright