Neonatal Exposure to Daidzein, Genistein, or the Combination Modulates Bone Development in Female CD-1 Mice
Neonatal Exposure to Daidzein, Genistein, or the Combination Modulates Bone Development in Female CD-1 Mice
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DOI：
10.3945/jn.108.100115
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发表时间：
2009-03-01
影响因子：
4.2
通讯作者：
Ward, Wendy E.
中科院分区：
文献类型：
--
作者：
Kaludjerovic, Jovana;Ward, Wendy E.
新生儿暴露于大豆中富含的染料木黄酮（GEN），有利于调节成年小鼠的骨矿物质密度（BMD）和骨强度。该研究的目的是确定早期暴露于天然存在于基于大豆蛋白的婴儿配方奶粉中的大豆黄酮大豆苷元（DAI）和GEN的组合是否比单独治疗对成年后的骨骼有更大的益处。将雄性和雌性CD-1小鼠（每组每种性别n = 8-16只幼仔）随机皮下注射DAI（mg.kg（-1. d（1））、GEN（mg.kg体重（1）-d（1））、DAI+GEN（7mg.kg体重（1）.d（1））、己烯雌酚（DES;阳性对照）（2mg.kg体重（1）.d（1））或对照（CON）从出生后第1-5天开始，并研究至4月龄。对股骨和腰椎（LV）的BMD、生物力学骨强度和骨微结构进行了评估。DAI、GEN、DAI+GEN或DES治疗组的女性LV BMD高于CON组（P < 0.05），DAI和DES组的椎骨对压缩性骨折的抵抗力更强。显微结构分析表明，与CON相比，DAI和GEN治疗分别导致更大（P < 0.05）的骨小梁连接性和骨小梁厚度。总之，新生儿暴露于DAI和/或GEN对成年雌性小鼠的骨骼有积极影响，但与单独治疗相比，DAI GEN对雌性或雄性小鼠的骨骼没有更大的益处。J. Nutr. 139：467-473，2009.
Neonatal exposure to genistein (GEN), an isoflavone abundant in soy, favorably modulates bone mineral density (BMD) and bone strength in mice at adulthood. The study objective was to determine whether early exposure to a combination of the soy isoflavones daidzein (DAI) and GEN that naturally exists in soy protein-based infant formula results in greater benefits to bone at adulthood than either treatment alone. Male and female CD-1 mice (n = 8-16 pups per group per gender) were randomized to Subcutaneous injections of DAI (2 mg.kg body weight(-1.)d(1)), GEN (5 mg.kg body weight (1)-d(1)), DAI+GEN (7 mg.kg body weight (1).d(1)), diethylstilbesterol (DES; positive control) (2 mg.kg body weight (1).d(1)), or control (CON) from postnatal d 1-5 and were studied to 4 mo of age. BMD, biomechanical bone strength, and bone microarchitecture were assessed at the femur and lumbar vertebrae (LV). Females treated with DAI, GEN, DAI+GEN, or DES had greater (P < 0.05) BMD at the LV compared with CON and vertebra in the DAI and DES group wore more resistant to compression fractures. Microstructural analyses demonstrated that treatment with DAI and GEN resulted in greater (P < 0.05) trabecular connectivity and trabecular thickness, respectively, than the CON. In conclusion, neonatal exposure to DAI and/or GEN had a positive effect on the skeleton of female mice at adulthood, but, compared with individual treatments, DAI GEN did riot have a greater benefit to bone in females or males. J. Nutr. 139: 467-473, 2009.