Male-Specific Transfer and Fine Scale Spatial Differences of Newly Identified Cuticular Hydrocarbons and Triacylglycerides in a Drosophila Species Pair
Male-Specific Transfer and Fine Scale Spatial Differences of Newly Identified Cuticular Hydrocarbons and Triacylglycerides in a Drosophila Species Pair
复制标题
果蝇物种对中新鉴定的表皮碳氢化合物和三酰甘油酯的雄性特异性转移和精细尺度空间差异
作者：
J. Yew;K. Dreisewerd;C. C. de Oliveira;W. Etges
我们分析了地理上孤立的种群D。mojavensis在不同的饲养基质上培养，并对其姊妹种D.用紫外激光解吸/电离质谱（UV-LDIMS）技术对亚利桑那州产的假单胞菌进行鉴定。不同的身体部位，即腿，长鼻，和鼻，这两个物种表现出定性相似的碳氢化合物的配置文件，主要由长链单烯，二烯，三烯，和四烯。然而，D. arizonae的大多数烃类含量高于D. mojavensis和这两个物种的女性表现出更大的碳氢化合物的量比男性。D.莫哈韦西斯种群的性别和饲养基板的显着影响，不同的身体部位。实验室食物饲养的苍蝇比发酵仙人掌基质饲养的苍蝇大多数碳氢化合物的含量低。我们发现了48个男性和物种特有的碳氢化合物，大小从C22到C50在男性肛门生殖器区域的两个物种，大多数没有描述过。这些包括几个含氧的碳氢化合物，除了高强度的信号对应于推定的甘油三酯，其量的幼虫饲养基板的影响。在交配过程中，这些化合物中的一些被转移到雌性库蚊中。这是第一个研究表明，三酰甘油酯可能是一个单独的类求爱相关的信号分子在果蝇。这项研究还扩展了这些物种的表皮碳氢化合物的种类和数量，并强调了幼虫生态学在影响这些化合物的数量中的作用，其中许多化合物在物种内和物种间介导求偶成功。
We analyzed epicuticular hydrocarbon variation in geographically isolated populations of D. mojavensis cultured on different rearing substrates and a sibling species, D. arizonae, with ultraviolet laser desorption/ionization mass spectrometry (UV-LDI MS). Different body parts, i.e. legs, proboscis, and abdomens, of both species showed qualitatively similar hydrocarbon profiles consisting mainly of long-chain monoenes, dienes, trienes, and tetraenes. However, D. arizonae had higher amounts of most hydrocarbons than D. mojavensis and females of both species exhibited greater hydrocarbon amounts than males. Hydrocarbon profiles of D. mojavensis populations were significantly influenced by sex and rearing substrates, and differed between body parts. Lab food–reared flies had lower amounts of most hydrocarbons than flies reared on fermenting cactus substrates. We discovered 48 male- and species-specific hydrocarbons ranging in size from C22 to C50 in the male anogenital region of both species, most not described before. These included several oxygen-containing hydrocarbons in addition to high intensity signals corresponding to putative triacylglycerides, amounts of which were influenced by larval rearing substrates. Some of these compounds were transferred to female cuticles in high amounts during copulation. This is the first study showing that triacylglycerides may be a separate class of courtship-related signaling molecules in drosophilids. This study also extends the kind and number of epicuticular hydrocarbons in these species and emphasizes the role of larval ecology in influencing amounts of these compounds, many of which mediate courtship success within and between species.
影响因子：
56.9
作者：
COYNE, JA;CRITTENDEN, AP;MAH, K
通讯作者：
MAH, K