Discrete genetic loci in human gutBacteroides thetaiotaomicron confer pectin metabolism
Discrete genetic loci in human gutBacteroides thetaiotaomicron confer pectin metabolism
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人肠道中的离散遗传位点多形拟杆菌赋予果胶代谢
DOI：
10.1016/j.carbpol.2021.118534
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发表时间：
2021-08-10
影响因子：
11.2
通讯作者：
Ding, Kan
中科院分区：
文献类型：
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作者：
Li, Meixia;Li, Saijuan;Ding, Kan
虽然果胶激活的多糖利用位点（普尔斯）已被确定，但由于果胶侧链结构复杂，其降解机制仍不清楚。因此，我们假设可能有其他特定的普尔斯靶向果胶。在这里，我们的特点位点编码的蛋白质表达的多形拟杆菌（BT）参与果胶捕获，进口，去分支和降解成单糖。共鉴定出4个普尔斯，其中包括10个酶和4个聚糖结合蛋白，包括一个新的表面酶和一个表面聚糖结合蛋白。值得注意的是，PUL2和PUL3迄今尚未报道。此外，我们表明，降解产物支持其他拟杆菌的生长。和益生菌此外，参与这一过程的基因在其他拟杆菌属中是保守的。我们的研究结果进一步强调了拟杆菌属的贡献。来代谢果胶网络。
Although the polysaccharide utilization loci (PULs) activated by pectin have been defined, due to the complex of side-chain structure, the degradative mechanisms still remain vague. Thus, we hypothesize that there may have other specific PULs to target pectin. Here, we characterize loci-encoded proteins expressed by Bacteroides thetaiotaomicron (BT) that are involved in the pectin capturing, importation, de-branching and degradation into monosaccharides. Totally, four PULs contain ten enzymes and four glycan binding proteins which including a novel surface enzyme and a surface glycan binding protein are identified. Notably, PUL2 and PUL3 have not been reported so far. Further, we show that the degradation products support the growth of other Bacteroides spp. and probiotics. In addition, genes involved in this process are conservative in other Bacteroides spp. Our results further highlight the contribution of Bacteroides spp. to metabolism the pectic network.