A planarian RPS3 homolog is critical to the modulation of planarian regeneration
A planarian RPS3 homolog is critical to the modulation of planarian regeneration
复制标题
涡虫 RPS3 同源物对于涡虫再生的调节至关重要。
DOI：
10.1016/j.gene.2018.12.027
复制
发表时间：
2019-04-05
期刊：
影响因子：
3.5
通讯作者：
Guo, Yanan
中科院分区：
文献类型：
--
作者：
Cheng, Xina;Li, Yingzhao;Guo, Yanan
涡虫是干细胞和再生生物学的重要模型，因为它们可以在短时间内再生任何缺失的身体结构。核糖体蛋白作为核糖体的重要组成部分，能够合成蛋白质，在细胞周期检查点、细胞存活/衰老/凋亡、机体生长发育等方面发挥核心作用。在本研究中，我们鉴定并扩增了杜鹃花RPS3的同源基因。双链RNA介导的RNAi显示，当涡虫失去Dj-RPS3功能时；它们没有形成胚泡，100%死亡。进一步研究证实，j- rps3参与调控新生细胞的增殖和早期分化。
Planarians are an important model for stem cell and regeneration biology, because they can regenerate any missing body structure in a short time. As an important component of ribosomes, ribosomal proteins can synthesize proteins and play a central role in cell cycle checkpoint, cell survival/senescence/apoptosis, and organismal growth and development. In this study, we identified and amplified the homologous gene of RPS3 in Dugesia japonica. Double-stranded RNA mediated RNAi revealed that when the Dj-RPS3 function was lost by planarians; they did not form blastemas and died 100%. Further investigation, confirmed that Dj-RPS3 was involved in regulating the proliferative and early differentiation of neoblasts.