Wheat alkylresorcinols protect human retinal pigment epithelial cells against H2O2-induced oxidative damage through Akt-dependent Nrf2/HO-1 signaling
Wheat alkylresorcinols protect human retinal pigment epithelial cells against H2O2-induced oxidative damage through Akt-dependent Nrf2/HO-1 signaling
复制标题
小麦烷基间苯二酚通过 Akt 依赖性 Nrf2/HO-1 信号传导保护人视网膜色素上皮细胞免受 H2O2 诱导的氧化损伤
DOI：
10.1039/c8fo02564a
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发表时间：
2019-05-01
期刊：
影响因子：
6.1
通讯作者：
Wang, Jing
中科院分区：
文献类型：
--
作者：
Wang, Ziyuan;Hao, Yiming;Wang, Jing
本研究探讨了小麦烷基间苯二酚（AR）对人视网膜色素上皮细胞（ARPE-19）抗氧化应激的保护作用及其可能的机制。结果表明，ARs显著抑制300 M H2 O2诱导的ARPE-19细胞损伤和活性氧（ROS）的产生，分别为19%和32%。此外，AR处理增加了NF-E2相关因子2（Nrf 2）信号传导激活，这通过增加抗氧化反应基因GCL、NQO 1和HO-1的转录来证明。通过靶向siRNA敲低Nrf 2可减轻ARs介导的HO-1转录，并几乎消除ARs介导的对H2 O2诱导的细胞损伤的细胞保护作用。进一步的研究表明，ARs的保护作用依赖于Akt的激活。综上所述，这些结果表明，AR可以保护ARPE-19细胞免受氧化应激诱导的细胞损伤，这可能是通过Akt依赖的Nrf 2/HO-1信号转导。
The protective effect of wheat alkylresorcinols (ARs) on human retinal pigment epithelium cells (ARPE-19) against oxidative stress and the possible underlying mechanism were investigated in this study. The results showed that ARs significantly inhibited 300 M H2O2-induced ARPE-19 cell damage and reactive oxygen species (ROS) generation by 19% and 32%, respectively. Moreover, ARs treatment increased NF-E2-related factor 2 (Nrf2) signaling activation, which was evidenced by increased transcription of anti-oxidant responsive genes GCL, NQO1 and HO-1. Knockdown of Nrf2 through targeted siRNA alleviated ARs-mediated HO-1 transcription, and almost abolished ARs-mediated cytoprotection against H2O2 induced cell damage. Further studies showed that the protective effect of ARs was dependent on Akt activation. Taken together, these results demonstrated that ARs could protect ARPE-19 cells from oxidative stress induced cell damage possibly through Akt dependent Nrf2/HO-1 signaling.