Precision Guided Sterile Males Suppress Populations of an Invasive Crop Pest
Precision Guided Sterile Males Suppress Populations of an Invasive Crop Pest
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DOI：
10.1089/genbio.2022.0019
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发表时间：
2022-08-01
期刊：
影响因子：
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通讯作者：
Akbari, Omar S.
中科院分区：
文献类型：
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作者：
Kandul, Nikolay P.;Liu, Junru;Akbari, Omar S.
铃木果蝇入侵西方国家，对农作物生产造成了巨大的农业和经济威胁。尽管多次试图抑制其种群，铃木丝虫仍在继续破坏软肉水果。精确引导不育昆虫技术(PgSIT)利用可编程CRISPR基因打靶的准确性来产生可用于抑制种群的不育雄性。在这里，我们在铃鹿中产生了pgSIT，并从经验和数学上证明了绝育的雄性是适合的、有竞争力的，并且可以消灭铃鹿的种群。总之，我们描述了一种产生不育铃木的有效方法，用于释放和安全有效的种群抑制。
The Drosophila suzukii invasion of western countries has created an immense agricultural and economic threat to crop production. Despite many attempts to suppress its population, D. suzukii continues to destroy soft-flesh fruits. Precision guided sterile insect technique (pgSIT) utilizes the accuracy of programmable CRISPR gene targeting to generate sterilized males that can be deployed to suppress populations. Here, we generate pgSIT in D. suzukii and empirically and mathematically demonstrate that sterilized males are fit, competitive, and can eliminate populations of D. suzukii. Altogether, we describe an efficient way to generate sterile D. suzukii for release and safe effective population suppression.