How replicable are mRNA expression QTL?
How replicable are mRNA expression QTL?
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DOI：
10.1007/s00335-005-0187-8
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发表时间：
2006-06-01
期刊：
影响因子：
2.5
通讯作者：
Lu, Lu
中科院分区：
文献类型：
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作者：
Peirce, Jeremy L.;Li, Hongqiang;Lu, Lu
将数量性状分析方法应用于分离群体中全基因组微阵列衍生的mRNA表达表型是试图将高水平性状与其分子原因联系起来的有价值的工具。在分析这些数据中涉及的大量多重检验问题使得在mRNA丰度数量性状基因座（QTL）中放置正确的置信水平成为一个难题。我们使用独特的资源来直接测试小鼠中mRNA丰度QTL的可复制性：来自C57 BL/6 J（B6）和DBA/2 J（D2）近交系的配对重组近交系（RI）和F-2数据集，并使用相同的Affytron阵列进行表型分析。我们有一个前脑和一个纹状体数据集对。我们描述了QTL复制在这些数据中不同的严格性。例如，78%的mRNA表达QTL（eQTL）具有全基因组校正的p
Applying quantitative trait analysis methods to genome-wide microarray-derived mRNA expression phenotypes in segregating populations is a valuable tool in the attempt to link high-level traits to their molecular causes. The massive multiple-testing issues involved in analyzing these data make the correct level of confidence to place in mRNA abundance quantitative trait loci (QTL) a difficult problem. We use a unique resource to directly test mRNA abundance QTL replicability in mice: paired recombinant inbred (RI) and F-2 data sets derived from C57BL/6J (B6) and DBA/2J (D2) inbred strains and phenotyped using the same Affymetrix arrays. We have one forebrain and one striatum data set pair. We describe QTL replication at varying stringencies in these data. For instance, 78% of mRNA expression QTL (eQTL) with genome-wide adjusted p