INACTIVATION OF POLYMERASE INHIBITORS FOR MYCOBACTERIUM-TUBERCULOSIS DNA AMPLIFICATION IN SPUTUM BY USING CAPTURE RESIN
INACTIVATION OF POLYMERASE INHIBITORS FOR MYCOBACTERIUM-TUBERCULOSIS DNA AMPLIFICATION IN SPUTUM BY USING CAPTURE RESIN
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DOI：
10.1128/jcm.33.3.629-630.1995
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发表时间：
1995-03-01
影响因子：
9.4
通讯作者：
SALTINI, C
中科院分区：
文献类型：
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作者：
AMICOSANTE, M;RICHELDI, L;SALTINI, C
内源性PCR抑制剂阻碍痰样本中结核分枝杆菌DNA的扩增。使用阴离子结合树脂（GeneReleaser [GR]）将PCR敏感性从单独酚-氯仿提取（P-C）的77%（35个培养阳性样品中的27个）提高到91%（GR不含P-C; P > 0.05）和100%（P-C和GR组合; P < 0.05）。
Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).