MOLECULAR EVOLUTION AND DISTRIBUTION OF DENGUE VIRUSES TYPE-1 AND TYPE-2 IN NATURE
MOLECULAR EVOLUTION AND DISTRIBUTION OF DENGUE VIRUSES TYPE-1 AND TYPE-2 IN NATURE
复制标题
DOI：
10.1016/0042-6822(90)90102-w
复制
发表时间：
1990-02-01
期刊：
影响因子：
3.7
通讯作者：
RICOHESSE, R
中科院分区：
文献类型：
--
作者：
RICOHESSE, R
在过去的几十年中，登革热病毒逐渐扩大了其地理分布，目前是与人类疾病相关的最重要的蚊媒病毒之一。确定这些RNA病毒的遗传变异性和传播模式对于制定有效的疾病控制策略至关重要。对< 3%的登革热基因组（跨越E/NS 1基因连接）进行引物延伸测序，为估计来自不同地理区域和宿主的40种登革热1型和40种2型病毒分离株之间的遗传关系提供了足够的信息。对这些240个核苷酸长的序列进行定量比较，揭示了以前未被认识到的疾病爆发之间的进化关系。五个不同的病毒基因型组检测到的两种血清型。流行性登革1型和2型病毒的进化速率相似，尽管这些病毒在世界各地的传播途径不同。对于2型登革热，一个基因型组代表了一个孤立的森林病毒循环，似乎在西非独立进化。这是第一个基因证据，证明登革热病毒存在森林循环，这与暴发病毒明显不同。
During the past several decades, dengue viruses have progressively extended their geographic distribution, and are currently some of the most important mosquito-borne viruses associated with human illness. Determining the genetic variability and transmission patterns of these RNA viruses is crucial in developing effective control strategies for the disease. Primer-extension sequencing of < 3% of the dengue genome (across the E/NS1 gene junction) provided sufficient information for estimating genetic relationships among 40 dengue type 1 and 40 type 2 virus isolates from diverse geographic areas and hosts. A quantitative comparison of these 240-nucleotide-long sequences revealed previously unrecognized evolutionary relationships between disease outbreaks. Five distinct virus genotypic groups were detected for each of the two serotypes. The evolutionary rates of epidemic dengue viruses of types 1 and 2 were similar, although the transmission pathways of these viruses around the world are different. For dengue type 2, one genotypic group represents an isolated, forest virus cycle which seems to have evolved independently in West Africa. This is the first genetic evidence of the existence of a sylvatic cycle of dengue virus, which is clearly distinct from outbreak viruses.