Screening of traditional Chinese medicines with therapeutic potential on chronic obstructive pulmonary disease through inhibiting oxidative stress and inflammatory response.
Screening of traditional Chinese medicines with therapeutic potential on chronic obstructive pulmonary disease through inhibiting oxidative stress and inflammatory response.
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DOI：
10.1186/s12906-016-1347-y
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发表时间：
2016-09-13
影响因子：
--
通讯作者：
Shen T
中科院分区：
文献类型：
--
作者：
Zhou MX;Wei X;Li AL;Wang AM;Lu LZ;Yang Y;Ren DM;Wang XN;Wen XS;Lou HX;Shen T
慢性阻塞性肺疾病（COPD）是一个严重的公共卫生问题，在世界范围内引起严重的慢性病发病率和死亡率。炎症反应和氧化应激在COPD的病理机制中起主导作用，被认为是COPD治疗的两个重要靶点。在我国中医药理论指导下，中药对慢性阻塞性肺疾病有较好的疗效，作为先导化合物资源值得深入研究。收集196种中药，提取并建立中药提取物库，然后分别采用NADP（H）：醌氧化还原酶（QR）法和一氧化氮（NO）产生法进一步评价其抑制氧化应激和炎症反应的效力。我们的研究观察到，38个测试的中药提取物诱导的QR活性在hepa 1c 1c 7小鼠肝癌细胞，其中55个抑制NO的产生在RAW 264.7小鼠巨噬细胞在测试的浓度。值得注意的是，20种中药提取物同时抑制氧化应激和炎症反应。所观察到的具有生物活性的中药，特别是这20种具有双重抑制作用的中药，可能对COPD的治疗有用。更重要的是，本研究的结果为我们提供了一个机会，发现新的铅分子作为COPD治疗药物从这些活性中药。本文的在线版本（doi：10.1186/s12906-016-1347-y）包含补充材料，可供授权用户使用。
Chronic obstructive pulmonary disease (COPD) is a major public health problem and gives arise to severe chronic morbidity and mortality in the world. Inflammatory response and oxidative stress play dominant roles in the pathological mechanism of COPD, and have been regarded to be two important targets for the COPD therapy. Traditional Chinese medicines (TCMs) possess satisfying curative effects on COPD under guidance of the TCM theory in China, and merit in-depth investigations as a resource of lead compounds. One hundred ninety-six of TCMs were collected, and extracted to establish a TCM extract library, and then further evaluated for their potency on inhibitions of oxidative stress and inflammatory response using NADP(H):quinone oxidoreductase (QR) assay and nitric oxide (NO) production assay, respectively. Our investigation observed that 38 of the tested TCM extracts induced QR activity in hepa 1c1c7 murine hepatoma cells, and 55 of them inhibited NO production in RAW 264.7 murine macrophages at the tested concentrations. Noteworthily, 20 of TCM extracts simultaneously inhibited oxidative stress and inflammatory responses. The observed bioactive TCMs, particularly these 20 TCMs with dual inhibitory effects, might be useful for the treatment of COPD. More importantly, the results of the present research afford us an opportunity to discover new lead molecules as COPD therapeutic agents from these active TCMs. The online version of this article (doi:10.1186/s12906-016-1347-y) contains supplementary material, which is available to authorized users.