Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis
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DOI：
10.1038/emboj.2012.346
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发表时间：
2013-03-06
期刊：
影响因子：
11.4
通讯作者：
Nasmyth, Kim
中科院分区：
文献类型：
--
作者：
Eichinger, Christian S.;Kurze, Alexander;Nasmyth, Kim
黏连蛋白的Smc 1、Smc 3和Kleisin亚基形成一个三环，在这个三环中，姐妹DNA被捕获。有证据表明，DNA通过Smc 1/3界面瞬时解离产生的门进入。后期开始时的释放由Kleisin的蛋白水解裂解触发。不太清楚的是在细胞周期的其他阶段释放的机制，特别是在前期，当大多数粘附素从染色体臂解离的过程中依赖于调节亚基Wapl。我们在这里表明，Wapl依赖的释放唾液腺多线染色体在间期和神经母细胞染色体臂在前期被阻止Smc 3的C-末端的Kleisin的N-末端的翻译融合。我们的研究结果表明，蛋白水解无关释放的粘附素从染色质介导的Wapl依赖逃逸的DNA通过门创建的短暂解离的Smc 3/kleisin接口。因此，粘着蛋白的DNA进入和退出门是不同的。The EMBO Journal（2013）32，656-665. doi：10.1038/daj.2012.346; 2013年1月22日在线发布
Cohesin's Smc1, Smc3, and kleisin subunits create a tripartite ring within which sister DNAs are entrapped. Evidence suggests that DNA enters through a gate created by transient dissociation of the Smc1/3 interface. Release at the onset of anaphase is triggered by proteolytic cleavage of kleisin. Less well understood is the mechanism of release at other stages of the cell cycle, in particular during prophase when most cohesin dissociates from chromosome arms in a process dependent on the regulatory subunit Wapl. We show here that Wapl-dependent release from salivary gland polytene chromosomes during interphase and from neuroblast chromosome arms during prophase is blocked by translational fusion of Smc3's C-terminus to kleisin's N-terminus. Our findings imply that proteolysis-independent release of cohesin from chromatin is mediated by Wapl-dependent escape of DNAs through a gate created by transient dissociation of the Smc3/kleisin interface. Thus, cohesin's DNA entry and exit gates are distinct. The EMBO Journal (2013) 32, 656-665. doi:10.1038/emboj.2012.346; Published online 22 January 2013