Enhanced initial adhesion of osteoblast-like cells on an anatase-structured titania surface formed by H2O2/HCl solution and heat treatment.
Enhanced initial adhesion of osteoblast-like cells on an anatase-structured titania surface formed by H2O2/HCl solution and heat treatment.
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DOI：
10.1016/j.dental.2008.09.007
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发表时间：
2009-04
期刊：
影响因子：
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通讯作者：
Xiao-feng Yang;Yao Chen;Fan Yang;F. He;Shi-fang Zhao
中科院分区：
文献类型：
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作者：
Xiao-feng Yang;Yao Chen;Fan Yang;F. He;Shi-fang Zhao
目的探讨锐钛矿型二氧化钛表面对成骨样细胞响应的影响。方法制备了3种钛片：抛光的钛(PT)、喷砂和酸蚀形成的粗糙钛(RT)以及经H_2O_2/HCl溶液处理后400℃加热形成的锐钛矿包覆的粗糙钛(ART)。扫描电子显微镜观察了钛片的表面形貌。将MC3T3-E1细胞接种于钛片上培养。用荧光显微镜观察细胞形态。用四甲基偶氮唑盐比色法检测细胞黏附和增殖情况。采用实时定量逆转录聚合酶链式反应检测核心结合因子α、Osterix、骨涎蛋白、I型胶原、骨钙素和碱性磷酸酶-2在RT和ART上的表达。ART上的细胞比PT和RT上的细胞更快地显示出明确的应力纤维和大量的突起和假足。培养后1h和3h，ART组的贴壁细胞数明显多于PT和RT组。培养1-5天后，各组间细胞增殖无差异。此外，与RT相比，ART普遍以时间依赖的方式促进成骨基因的表达。信号：锐钛矿型结构的二氧化钛促进了成骨细胞的初始黏附和铺展，但不影响细胞的增殖。锐钛矿还可以通过促进成骨基因的表达来促进细胞分化。
OBJECTIVESThis study aims to investigate the effects of anatase-structured titania surface on the response of osteoblast-like cells.METHODSThree kinds of titanium (Ti) disks were prepared: the polished Ti (PT), the roughened Ti (RT) formed by sandblasting and acid etching, and the anatase-coated roughened Ti (ART) formed on the RT via H2O2/HCl solution treatment followed by heating at 400°C. The surface topography of Ti disks was observed under a scanning electron microscope. MC3T3-E1 cells were cultured on the Ti disks. Cell morphology was observed by fluorescence microscopy. Cell adhesion and proliferation were assessed by MTT testing. To evaluate the cell differentiation on RT and ART, the expressions of core-binding factor α, osterix, bone sialoprotein, collagen type I????, osteocalcin and alkaline phosphatase-2 were measured by real-time reverse transcriptase polymerase chain reaction.RESULTSThe three kinds of Ti disks had distinct surface topographic characteristics. The cells on the ART more rapidly displayed well-defined stress fibers and numerous processes and pseudopodi than the cells on the PT and RT. At 1 and 3h after culture, there were more cells adhering to the ART than to PT and RT. Cell proliferation did not differ between groups after 1–5 days of culture. In addition, ART generally enhanced the osteogenic gene expression in a time-dependent manner compared with RT.SIGNIFICANCEAnatase-structured titania promotes initial adhesion and spreading of osteoblast-like cells, but does not influence cell proliferation. Anatase could also enhance cell differentiation by enhancing osteogenic gene expression.