Renal sodium transport in renin-deficient Dahl salt-sensitive rats.
Renal sodium transport in renin-deficient Dahl salt-sensitive rats.
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DOI：
10.1177/1470320316653858
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发表时间：
2016-07
期刊：
影响因子：
--
通讯作者：
Staruschenko A
中科院分区：
文献类型：
--
作者：
Pavlov TS;Levchenko V;Ilatovskaya DV;Moreno C;Staruschenko A
Dahl盐敏感大鼠是盐敏感性高血压的成熟模型。本研究的目的是评估肾钠通道和转运蛋白的表达和活性在肾素缺乏盐敏感大鼠。在盐敏感大鼠背景下建立的肾素基因敲除（Ren-/-）大鼠用于研究肾素在盐敏感性高血压中离子转运调节的作用。采用Western blotting和膜片钳技术检测Na+转运蛋白的表达水平和活性。之前已经描述过，Ren−/−大鼠表现出严重的肾脏发育不全，多尿，体重和血压低于野生型同窝仔。在这里，我们发现，肾素缺乏导致钠-氢逆向转运蛋白（NHE 3）的表达减少，钠/H+交换参与钠吸收在近端小管，但不影响钠-钾-氯协同转运蛋白（NKCC 2）的表达，主要转运蛋白在亨利环。在远端肾单位，氯化钠协同转运蛋白（NCC）的表达在Ren−/−大鼠中较低。单通道膜片钳分析检测到Ren−/−大鼠的ENaC活性降低，这是通过通道开放概率的变化介导的。这些数据说明，肾素缺乏导致离子转运蛋白的显著失调。
The Dahl salt-sensitive rat is a well-established model of salt-sensitive hypertension. The goal of this study was to assess the expression and activity of renal sodium channels and transporters in the renin-deficient salt-sensitive rat. Renin knockout (Ren−/−) rats created on the salt-sensitive rat background were used to investigate the role of renin in the regulation of ion transport in salt-sensitive hypertension. Western blotting and patch-clamp analyses were utilized to assess the expression level and activity of Na+ transporters. It has been described previously that Ren−/− rats exhibit severe kidney underdevelopment, polyuria, and lower body weight and blood pressure compared to their wild-type littermates. Here we found that renin deficiency led to decreased expression of sodium-hydrogen antiporter (NHE3), the Na+/H+ exchanger involved in Na+ absorption in the proximal tubules, but did not affect the expression of Na-K-Cl cotransporter (NKCC2), the main transporter in the loop of Henle. In the distal nephron, the expression of sodium chloride cotransporter (NCC) was lower in Ren−/− rats. Single-channel patch clamp analysis detected decreased ENaC activity in Ren−/− rats which was mediated via changes in the channel open probability. These data illustrate that renin deficiency leads to significant dysregulation of ion transporters.
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影响因子：
13.6
作者：
Pavlov, Tengis S.;Chandi, Ahmed;Staruschenko, Alexander
通讯作者：
Staruschenko, Alexander
影响因子：
8.3
作者：
Ares, Gustavo R.;Haque, Mohammed Z.;Ortiz, Pablo A.
通讯作者：
Ortiz, Pablo A.
DOI：
10.1152/ajprenal.00422.2005
发表时间：
2006-09-01
影响因子：
4.2
作者：
Ergonul, Zuhal;Frindt, Gustavo;Palmer, Lawrence G.
通讯作者：
Palmer, Lawrence G.
影响因子：
1.2
作者：
Pavlov, Tengis S.;Ilatovskaya, Daria V.;Staruschenko, Alexander
通讯作者：
Staruschenko, Alexander
DOI：
10.1073/pnas.1201978109
发表时间：
2012-06-19
影响因子：
11.1
作者：
Mironova, Elena;Bugaj, Vladislav;Stockand, James D.
通讯作者：
Stockand, James D.