Automated determination of nitrite in aqueous samples with an improved integrated flow loop analyzer
Automated determination of nitrite in aqueous samples with an improved integrated flow loop analyzer
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使用改进的集成流动回路分析仪自动测定水样中的亚硝酸盐
DOI：
10.1016/j.snb.2016.07.002
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发表时间：
2016-12-01
影响因子：
8.4
通讯作者：
Ma, Jian
中科院分区：
文献类型：
--
作者：
Liu, Baomin;Su, Haitao;Ma, Jian
基于Griess反应和分光光度检测原理，研制了一种用于水样中亚硝酸盐自动测定的小型环流分析仪。该集成系统采用定制的基于单片机的硬件和由LabVIEW编写的实验室编程软件来控制注射阀、泵和光学检测模块。对亚硝酸盐分析的相关实验参数，包括试剂体积、样品温度和盐度进行了评价和优化。该全自动分析仪的检测限低至0.02 μ M，样品通量为15 h（-1）。在纯水和低营养盐海水中制备的校准曲线在0-10 μ M的线性范围内一致（R-2 = 0.999），显示海水中常见离子的干扰不明显。使用本分析仪和标准台式方法成功地分析了不同的水样，根据统计学t检验，两种技术的结果显示无显著差异（P=0.95）。这款紧凑型自动分析仪结构简单、完全集成、用户友好，非常适合现场应用，如在线环境监测和正在进行的分析。(C)2016爱思唯尔B. V.保留所有权利。
A compact loop flow analyzer was developed for automated determination of nitrite in aqueous samples based on Griess reaction and spectrophotometric detection. The integrated system employed a customized single chip microcomputer based hardware and laboratory-programmed software written by LabVIEW to control the injection valve, pumps and optical detection module. Experimental parameters related to nitrite analysis, including the reagent volume, sample temperature and salinity were evaluated and optimized. This fully automated analyzer showed a limit of detection as low as 0.02 mu M with sample throughput of 15 h(-1). The calibration curves prepared in pure water and low nutrient seawater were consistent over the linear range of 0-10 mu M (R-2 = 0.999), showing insignificant interference from the common ions in seawater. Different aqueous samples were successfully analyzed using both the present analyzer and the standard benchtop method, and the results from the two techniques showed no significant difference according to the statistical t-test (P=0.95). This compact automatic analyzer is simple, fully integrated, and user-friendly, making it highly suitable for field applications, such as on-line environmental monitoring and underway analysis. (C) 2016 Elsevier B.V. All rights reserved.