Functional differentiation of peroxisomes revealed by expression profiles of peroxisomal genes in Arabidopsis thaliana
Functional differentiation of peroxisomes revealed by expression profiles of peroxisomal genes in Arabidopsis thaliana
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DOI：
10.1093/pcp/pcg173
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发表时间：
2003-12-01
影响因子：
4.9
通讯作者：
Nishimura, M
中科院分区：
文献类型：
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作者：
Kamada, T;Nito, K;Nishimura, M
众所周知，过氧化物酶体基质蛋白含有两种靶向信号PTS 1和PTS 2之一。我们全面调查了整个拟南芥基因组序列中与过氧化物酶体功能和生物发生相关的基因。在这里，我们确定了256个候选基因的PTS 1-和PTS 2-含有蛋白质和另外30个基因的非PTS-含有蛋白质。其中，只有29种蛋白质已被报道在高等植物中具有过氧化物酶体蛋白的功能特征。我们广泛研究了上述基因在拟南芥不同器官中的表达谱。这些表达谱的统计分析表明，过氧化物酶体基因可以分为五组。一组在所有检查的器官中表现出普遍的表达，而其他四个被归类为在幼苗、子叶、根以及子叶和叶中表现出器官特异性表达。这些数据为植物过氧化物酶体的分化提供了更详细的描述。
It is well known that peroxisomal matrix proteins contain one of two targeting signals, PTS1 and PTS2. We comprehensively surveyed genes related to peroxisomal function and biogenesis in the entire Arabidopsis genome sequence. Here, we identified 256 gene candidates of PTS1- and PTS2-containing proteins and another 30 genes of non-PTS-containing proteins. Of these, only 29 proteins have been reported to be functionally characterized as peroxisomal proteins in higher plants. We extensively investigated expression profiles of genes described above in various organs of Arabidopsis. Statistical analyses of these expression profiles revealed that peroxisomal genes could be divided into five groups. One group showed ubiquitous expression in all organs examined, while the other four were classified as showing organ-specific expression in seedlings, cotyledons, roots and in both cotyledons and leaves. These data proposed more detailed description of differentiation of plant peroxisomes.