Automated low dose assay system for survival measurements of mammalian cells in vitro
Automated low dose assay system for survival measurements of mammalian cells in vitro
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用于体外哺乳动物细胞存活测量的自动化低剂量测定系统
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
L. Skarsgard
中科院分区：
文献类型：
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作者：
B. Palcic;B. Faddegon;B. Jaggi;L. Skarsgard
为了研究低剂量电离辐射对细胞的影响，我们开发了自动低剂量检测系统，这是一个半自动的计算机辅助系统，可以对单个细胞进行有效的检查。具体地说，它提供了系统和可靠的细胞位置定位，并具有返回到这些位置以便对细胞生长进行分类的手段。该系统允许以12.5-微米步长对7×12厘米表面进行显微扫描，重复性为100微米。
To study the effects of low dose ionizing radiation on cells, we developed the automated low dose assay systems which is a semiautomatic computer aided system that permits efficient examination of individual cells. Specifically, it provides for systematic and reliable location of cell positions and it features the means to return to those positions in order to classify cell growth. The system allows for microscopic scanning of a 7 × 12 cm surface in 12.5-µm steps with a repeatability of 100 µm.