Improved solubilization procedures for liquid scintillation counting of biological materials
Improved solubilization procedures for liquid scintillation counting of biological materials
复制标题
改进生物材料液体闪烁计数的溶解程序
DOI：
10.1016/0003-2697(67)90016-4
复制
发表时间：
1967
影响因子：
2.9
通讯作者：
E. Bush
中科院分区：
文献类型：
--
作者：
D. L. Hansen;E. Bush
氢氧化季铵盐和NCS似乎是能够溶解软组织和许多纯化的生物材料，使低能量β发射体（H3和C14）可以计算与真正的解决方案的效率，确定通过内部标准化，通道比等酒精KOH不。NCS获得了最高的品质因数，在许多情况下，这些与燃烧技术可获得的品质因数相比是有利的。描述了改进的消化、漂白和稀释程序。
Hyamine hydroxide and NCS appear to be capable of solubilizing soft tissues and many purified biological materials so that low-energy beta emitters (H3and C14) can be counted with true solution efficiencies, as determined by internal standardization, channels ratio, etc. Alcoholic KOH does not. Highest figures of merit are obtained with NCS, and in a number of cases these compare favorably with those attainable by combustion techniques. Improved digestion, bleaching, and dilution procedures are described.