Tracking of migrating cells under phase-contrast video microscopy with combined mean-shift processes
Tracking of migrating cells under phase-contrast video microscopy with combined mean-shift processes
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DOI：
10.1109/tmi.2005.846851
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发表时间：
2005-06-01
影响因子：
10.6
通讯作者：
Decaestecker, C
中科院分区：
文献类型：
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作者：
Debeir, O;Van Ham, P;Decaestecker, C
在本文中，我们提出了一种基于平均位移的跟踪过程的组合，通过体外相衬视频显微镜建立迁移细胞轨迹。在概述均值移位算法如何允许有效的目标跟踪之后，我们描述了所提出的扩展并将其应用于体外细胞跟踪问题。在这种应用中，细胞是未标记的（即，没有使用荧光探针），并在经典的相衬显微镜下观察。通过引入几个核的自适应组合，我们解决了几个问题，如跟踪对象的大小和形状的变化（例如，在细胞膜延伸的情况下发生的变化），不完整（或非对比）对象边界的存在，部分重叠的对象和对象分裂（在细胞分裂或有丝分裂的情况下）。将自动获得的跟踪结果与人工专家生成的跟踪结果进行比较，测试了不同算法参数的稳定性及其对跟踪结果的影响。我们还展示了该方法如何抵抗图像分辨率下降和意外散焦（这可能发生在长时间的实验中，例如，几十小时）。最后，我们将我们的方法应用于癌细胞跟踪，并表明细胞松弛素d显着抑制细胞运动。
In this paper, we propose a combination of mean-shift-based tracking processes to establish migrating cell trajectories through in vitro phase-contrast video microscopy. After a recapitulation on how the mean-shift algorithm permits efficient object tracking we describe the proposed extension and apply it to the in vitro cell tracking problem. In this application, the cells are unmarked (i.e., no fluorescent probe is used) and are observed under classical phase-contrast microscopy. By introducing an adaptive combination of several kernels, we address several problems such as variations in size and shape of the tracked objects (e.g., those occurring in the case of cell membrane extensions), the presence of incomplete (or noncontrasted) object boundaries, partially overlapping objects and object splitting (in the case of cell divisions or mitoses). Comparing the tracking results automatically obtained to those generated manually by a human expert, we tested the stability of the different algorithm parameters and their effects on the tracking results. We also show how the method is resistant to a decrease in image resolution and accidental defocusing (which may occur during long experiments, e.g., dozens of hours). Finally, we applied our methodology on cancer cell tracking and showed that cytochalasin-D significantly inhibits cell motility.