INDIVIDUAL-DIFFERENCES IN AGING - BEHAVIORAL AND NEUROBIOLOGICAL CORRELATES
INDIVIDUAL-DIFFERENCES IN AGING - BEHAVIORAL AND NEUROBIOLOGICAL CORRELATES
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DOI：
10.1016/s0197-4580(89)80008-9
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发表时间：
1989-01-01
影响因子：
4.2
通讯作者：
OLTON, DS
中科院分区：
文献类型：
--
作者：
MARKOWSKA, AL;STONE, WS;OLTON, DS
本实验的目的是确定老年大鼠不同行为和神经生物学指标之间的相关性。对老年Sprague-Dawley大鼠进行一系列认知和感觉运动测试，然后进行睡眠电生理评估和各种神经递质系统的生化测量。行为测试包括以下内容：在开阔场地的活动水平;通过习惯化评估的空间环境的短期记忆;空间导航、辨别反转和Morris水池中的线索学习;由从水中逃脱激发的T型迷宫中的空间记忆;巴恩斯圆形平台任务的空间记忆和反转;被动回避;运动技能。睡眠通过皮层电图记录进行评估。检查了以下神经递质标志物：胆碱乙酰转移酶;皮质和尾状核中烟碱、苯二氮卓类和谷氨酸受体的密度;皮质和海马中去甲肾上腺素、多巴胺和5-羟色胺的内源性水平。矛盾睡眠发作的持续时间与几种认知测量和选定的多巴胺能标记物密切相关。这一发现表明，睡眠模式和大脑生物化学的变化直接导致学习和记忆的缺陷，或者同样的神经生物学缺陷导致与年龄相关的睡眠和学习记忆障碍。
The goal of this experiment was to determine the correlations among different behavioral and neurobiological measures in aged rats. Aged Sprague-Dawley rats were given a battery of cognitive and sensorimotor tests, followed by electrophysiological assessment of sleep and biochemical measurements of various neurotransmitter systems. The behavioral tests included the following: Activity level in an open field; short-term memory of a spatial environment as assessed by habituation; spatial navigation, discrimination reversal, and cue learning in the Morris water pool; spatial memory in a T-maze motivated by escape from water; spatial memory and reversal on the Barnes circular platform task; passive avoidance; motor skills. Sleep was assessed by electrographic cortical records. The following neurotransmitter markers were examined: Choline acetyltransferase; the density of nicotinic, benzodiazepine, and glutamate receptors in the cortex and caudata nucleus; endogenous levels of norepinephrine, dopamine, and serotonin in the cortex and hippocampus. The duration of bouts of paradoxical sleep was strongly correlated with several cognitive measures and selected serotonergic markers. This finding suggests that changes in sleep patterns and brain biochemistry contribute directly to deficites in learning and memory, or that the same neurobiological defect contributes to age-related impairments in sleep and in learning and memory.