DIRECT RADIOIMMUNOASSAY OF ATRIAL NATRIURETIC FACTOR
DIRECT RADIOIMMUNOASSAY OF ATRIAL NATRIURETIC FACTOR
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DOI：
10.1016/s0006-291x(84)80074-1
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
GENEST, J
中科院分区：
文献类型：
--
作者：
GUTKOWSKA, J;THIBAULT, G;GENEST, J
建立了心房利钠因子（ANF）的直接放射免疫测定方法。该方法利用天然肽的26个氨基酸片段（8-33 ANF）合成。用该肽偶联甲状腺球蛋白免疫家兔制备了抗体。采用高效液相色谱法对氯胺- t碘化法制备的放射性示踪剂进行纯化，并对Sepharose-4B anti-ANF进行亲和层析。右旋糖酐包被炭已被用于游离抗体结合放射性的分离。大鼠右心房ANF含量高于左心房。这些结果经生物试验证实。
A direct radioimmunoassay of atrial natriuretic factor (ANF) was developed. The method uses a synthetic 26 amino-acid fragment (8-33 ANF) of the native peptide. Antibodies have been prepared in rabbits immunized with the peptide coupled to thyroglobulin. The radiolabeled tracer prepared by iodination according to the Chloramine-T method has been purified by HPLC [high performance liquid chromatography] followed by affinity chromatography on Sepharose-4B anti-ANF. Dextran-coated charcoal has been used for separation of free from antibody bound radioactivity. Higher ANF content was found in the right rat atrium than in the left. These results have confirmed by bioassay.