Modulation of fibroblast growth by a lymphokine of human T cell continuous T cell line origin.
Modulation of fibroblast growth by a lymphokine of human T cell continuous T cell line origin.
复制标题
人 T 细胞连续 T 细胞系来源的淋巴因子对成纤维细胞生长的调节。
作者：
S. Wahl;C. Gately
人外周血T淋巴细胞被有丝分裂原或特异性抗原激活产生一种介质，刺激静止的真皮成纤维细胞群增殖。这种淋巴细胞产物可能与慢性细胞介导的炎性病变相关的纤维化有关，但未受刺激的淋巴细胞不会产生这种产物。成纤维细胞促有丝分裂因子的特征将其与具有类似活性的单核细胞衍生因子区分开来。T细胞因子似乎是一种具有明显分子量的蛋白质。40，000和5.0和5.5之间的等电点。这种成纤维细胞生长因子可以在相对缺乏单核细胞的情况下产生，并且该因子是T细胞产物的进一步证据源于人T细胞系产生这种因子的能力。正常T细胞产物的生化特性与T细胞系的生化特性的比较揭示它们是不可区分的。因此，T细胞系可以用作成纤维细胞活化因子（FAF）的来源，以更详细地分析其结构和功能。这种淋巴因子的进一步表征可能有助于理解在正常组织修复以及与某些慢性炎症性疾病相关的病理生理纤维化反应中介导纤维化的机制。
Human peripheral blood T lymphocytes activated by mitogens or specific antigen produce a mediator that stimulates proliferation in a quiescent population of dermal fibroblasts. This lymphocyte product, which may have implications in the fibrosis associated with chronic cell-mediated inflammatory lesions, is not elaborated by unstimulated lymphocytes. Characterization of the fibroblast mitogenic factor differentiated it from a monocyte-derived factor with similar activity. The T cell factor appears to be a protein with an apparent m.w. of 40,000 and an isoelectric point between 5.0 and 5.5. This fibroblast growth factor can be produced in the relative absence of monocytes, and further evidence that the factor is a T cell product stems from the ability of a human T cell line to generate such a factor. Comparison of the biochemical characteristics of the normal T cell product with those of the T cell line revealed them to be indistinguishable. Thus, the T cell line can be used as a source of fibroblast-activating factor (FAF) for more detailed analysis of its structure and function. Further characterization of this lymphokine may contribute to understanding the mechanisms that mediate fibrosis in normal tissue repair as well as in the pathophysiologic fibrotic response associated with certain chronic inflammatory diseases.