Detection and localization of two hydrogenases in Methylococcus capsulatus (Bath) and their potential role in methane metabolism
Detection and localization of two hydrogenases in Methylococcus capsulatus (Bath) and their potential role in methane metabolism
复制标题
DOI：
10.1007/s00203-001-0372-4
复制
发表时间：
2002-02-01
影响因子：
2.8
通讯作者：
Kovács, KL
中科院分区：
文献类型：
--
作者：
Hanczár, T;Csáki, R;Kovács, KL
荚膜甲基球菌（Methylococcus capsulatus，Bath）含有两种不同的氢化酶，一种是可溶性氢化酶，另一种是膜结合型氢化酶。这是甲烷氧化菌中膜结合氢化酶的首次报道。这两种酶在正常生长条件下明显组成型表达。可溶性氢化酶能够用分子氢还原NAD（+）。可溶性甲烷单加氧酶和颗粒状甲烷单加氧酶的活性均受分子氢驱动。这证实了分子氢可以用作甲烷氧化的还原力来源。氢驱动的甲烷单加氧酶活性耐受升高的温度和中等的氧浓度。这些研究结果的甲烷氧化菌的生物技术应用的意义进行了讨论。
Methylococcus capsulatus (Bath) was shown to contain two distinct hydrogenases, a soluble hydrogenase and a membrane-bound hydrogenase. This is the first report of a membrane-bound hydrogenase in methanotrophs. Both enzymes were expressed apparently constitutively under normal growth conditions. The soluble hydrogenase was capable of reducing NAD(+) with molecular hydrogen. The activities of both soluble and particulate methane monooxygenases could be driven by molecular hydrogen. This confirmed that molecular hydrogen could be used as a source of reducing power for methane oxidation. Hydrogen-driven methane monooxygenase activities tolerated elevated temperatures and moderate oxygen concentrations. The significance of these findings for biotechnological applications of methanotrophs is discussed.