Phosphorylation of five histone H1 subtypes of L5178Y cells at the exponential growth and mitotic phases.
Phosphorylation of five histone H1 subtypes of L5178Y cells at the exponential growth and mitotic phases.
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L5178Y 细胞的五种组蛋白 H1 亚型在指数生长期和有丝分裂期的磷酸化。
DOI：
10.1093/oxfordjournals.jbchem.a135327
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发表时间：
1985
影响因子：
2.7
通讯作者：
Y. Ohba
中科院分区：
文献类型：
--
作者：
T. Matsukawa;H. Adachi;Y. Kurashina;Y. Ohba
小鼠淋巴细胞白血病细胞系L5178Y的组蛋白H1由5种分子组成，命名为H1-I、II、III、IV和V。在指数生长期和有丝分裂期间，用BioRex 70柱层析和双向聚丙烯酰胺凝胶电泳法检测了这些H1亚型的磷酸化。在指数生长的细胞中，每种亚型的磷酸化程度都不同。H1-II是磷酸化程度最高的，每个分子1.8个磷酸残基，其次是H1-IV/V，1.4，I，0.8，III，0.5。在有丝分裂阶段，H1-II也是每个分子中磷酸化程度最高的6.0个磷酸残基，H1-IV/V为3.5，I，2.7，III，1.2。秋水仙素加入后，各亚型间的磷酸化同时开始，磷酸化的H1亚型呈线性积累。在秋水仙胺治疗期间，~(32)P掺入各H1亚型的速率几乎是恒定的。在加入秋水仙胺后的4h内，H1中的磷酸盐残基没有去磷酸化。在秋水仙素治疗期间，H1激酶活性增加到6倍。
Histone H1 of cells of L5178Y, a mouse lympholeukemic cell line, consists of five molecular species designated as H1-I, II, III, IV, and V. The phosphorylation of these H1 subtypes was examined at the exponential growth phase and during mitosis, by BioRex 70 column chromatography and two-dimensional polyacrylamide gel electrophoresis. In exponentially growing cells, the degree of phosphorylation was different for each subtype. H1-II was the most highly phosphorylated, 1.8 phosphate residues per molecule, followed by H1-IV/V, 1.4, I, 0.8, and III, 0.5. In the mitotic phase, H1-II was also the most highly phosphorylated 6.0 phosphate residues per molecule, H1-IV/V, 3.5, I, 2.7, and III, 1.2. The phosphorylation started simultaneously among the subtypes after colcemid addition, and phosphorylated H1 subtypes accumulated linearly. The rate of incorporation of 32P into each H1 subtype was almost constant during colcemid treatment. During 4 h after colcemid addition, the phosphate residues incorporated into H1 did not dephosphorylated. The H1 kinase activities increased to six times higher during the colcemid treatment.