Acid- and Thiol-Cleavable Multifunctional Codelivery Hydrogel: Fabrication and Investigation of Antimicrobial and Anticancer Properties.
Acid- and Thiol-Cleavable Multifunctional Codelivery Hydrogel: Fabrication and Investigation of Antimicrobial and Anticancer Properties.
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DOI：
10.1021/acsabm.0c01396
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发表时间：
2021-02
影响因子：
4.7
通讯作者：
Jie Zha;Xiao-Xu Mao;Shoukui Hu;Ke Shang;Jun Yin
中科院分区：
文献类型：
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作者：
Jie Zha;Xiao-Xu Mao;Shoukui Hu;Ke Shang;Jun Yin
水凝胶作为药物载体是通过将小尺寸药物分子捕获在其交联的间隙网络中而实现的。覆盖目标位置后，水凝胶与生理液体相互作用并膨胀，导致网络之间的间隙增加，以便药物向外扩散。然而，植入材料上不可避免的体内炎症反应或细菌感染以及持续的货物释放仍然具有挑战性。在此，我们报道了通过传统自由基聚合基于酸敏感的聚（乙烯亚胺）（PEI）衍生物（PEI（-COOH/-vinyl））、硫醇响应性喜树碱前药单体（CPTM）和亲水性低聚（乙二醇）甲醚丙烯酸酯（OEGMA）的双响应水凝胶的制备。然后将姜黄素溶解到水凝胶中，赋予它们抗菌和抗癌特性。体外实验在模拟生理环境中表现出持续的水凝胶溶解和喜树碱释放。分别使用耐甲氧西林金黄色葡萄球菌（MRSA）菌株和HeLa癌细胞系对载药水凝胶进行抗菌和细胞毒性测试，表明该水凝胶具有高效的抗菌作用，并能成功抑制癌细胞的生长。
Hydrogels serving as a drug carrier was realized by entrapping small-sized drug molecules within their cross-linked interstitial networks. After covering the targeted location, hydrogels interact with the physiological fluids and swell, resulting in an increased interspace between networks for the outside diffusion of drugs. However, inevitable in vivo inflammatory responses or bacterial infection on the implant materials and persistent cargo release are still challenging. Herein, we report the fabrication of dual-responsive hydrogels based on acid-sensitive poly(ethylenimine) (PEI) derivative (PEI(-COOH/-vinyl)), thiol-responsive camptothecin prodrug monomer (CPTM), and hydrophilic oligo(ethylene glycol) methyl ether acrylate (OEGMA) by a conventional radical polymerization. Curcumin was then solubilized into the hydrogels to endow them with antimicrobial and cancer resistance properties. The in vitro experiments exhibited sustained hydrogel dissolution and CPT release in a simulated physiological environment. The antimicrobial and cytotoxicity tests of drug-loaded hydrogels using methicillin-resistant Staphylococcus aureus (MRSA) strains and HeLa cancer cell lines, respectively, indicated that the hydrogels possessed efficient antimicrobial effects and could successfully inhibit the growth of cancer cells.