Simultaneous nano-tracking of multiple motor proteins via spectral discrimination of quantum dots
Simultaneous nano-tracking of multiple motor proteins via spectral discrimination of quantum dots
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DOI：
10.1364/boe.7.002475
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发表时间：
2016-07-01
影响因子：
3.4
通讯作者：
Ichimura, Taro
中科院分区：
文献类型：
--
作者：
Kakizuka, Taishi;Ikezaki, Keigo;Ichimura, Taro
通过将靶蛋白的多色荧光标记与成像光谱相结合，实现了对多种马达蛋白的同时纳米跟踪，在亚衍射极限尺度下揭示了多种马达蛋白的动态行为。利用不同颜色的量子点探针，我们实验验证了定位精度为几纳米，时间分辨率为30毫秒或更快。成功地跟踪了单个肌凝蛋白分子和多个肌凝蛋白分子的两个头部的一维过程运动。此外，该系统被改进为二维测量，并应用于多个肌凝蛋白分子的跟踪。该方法可用于研究超分子纳米机械中蛋白质的协同运动。(C) 2016年美国光学学会
Simultaneous nanometric tracking of multiple motor proteins was achieved by combining multicolor fluorescent labeling of target proteins and imaging spectroscopy, revealing dynamic behaviors of multiple motor proteins at the sub-diffraction-limit scale. Using quantum dot probes of distinct colors, we experimentally verified the localization precision to be a few nanometers at temporal resolution of 30 ms or faster. One-dimensional processive movement of two heads of a single myosin molecule and multiple myosin molecules was successfully traced. Furthermore, the system was modified for two-dimensional measurement and applied to tracking of multiple myosin molecules. Our approach is useful for investigating cooperative movement of proteins in supramolecular nanomachinery. (C) 2016 Optical Society of America