Poly(A)-independent regulation of maternal hunchback translation in the Drosophila embryo
Poly(A)-independent regulation of maternal hunchback translation in the Drosophila embryo
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DOI：
10.1073/pnas.201284398
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发表时间：
2001-09-25
影响因子：
11.1
通讯作者：
Lehmann, R
中科院分区：
文献类型：
--
作者：
Chagnovich, D;Lehmann, R
果蝇腹部的发育需要抑制胚胎后部母体驼背（hb） mRNA的翻译。该调控涉及至少四个组成部分：hb 3 '非翻译区的nanos应答元件和Pumilio （PUM）、nanos （NOS）和脑肿瘤的活性。为了研究这种调节，我们开发了一种RNA注射试验，忠实地概括了内源性hb信息的调节。先前的研究表明，NOS和PUM可以通过指导poly(A)的去除来调节翻译。我们发现缺乏聚(a)尾的rna不能被聚腺苷化，而含有翻译激活序列代替聚(a)尾的rna在后部仍然受到抑制。这些数据表明，多聚(A)尾不是调控所必需的，NOS和PUM可以通过两种机制调控hb翻译：去除多聚(A)尾和直接影响翻译的多聚(A)独立通路。
Development of the Drosophila abdomen requires repression of maternal hunchback (hb) mRNA translation in the posterior of the embryo. This regulation involves at least four components: nanos response elements within the hb 3 ' untranslated region and the activities of Pumilio (PUM), Nanos (NOS), and Brain tumor. To study this regulation, we have developed an RNA injection assay that faithfully recapitulates the regulation of the endogenous hb message. Previous studies have suggested that NOS and PUM can regulate translation by directing poly(A) removal. We have found that RNAs that lack a poly(A) tail and cannot be polyadenylated and RNAs that contain translational activating sequences in place of the poly(A) tail are still repressed in the posterior. These data demonstrate that the poly(A) tail is not required for regulation and suggest that NOS and PUM can regulate hb translation by two mechanisms: removal of the poly(A) tail and a poly(A)-independent pathway that directly affects translation.