3D micro-particle image modeling and its application in measurement resolution investigation for visual sensing based axial localization in an optical microscope
3D micro-particle image modeling and its application in measurement resolution investigation for visual sensing based axial localization in an optical microscope
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3D 微粒图像建模及其在光学显微镜中基于视觉传感的轴向定位测量分辨率研究中的应用
DOI：
10.1088/1361-6501/28/1/015402
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发表时间：
2016
影响因子：
2.4
通讯作者：
Liu, Jinhua
中科院分区：
文献类型：
--
作者：
Li, Xiaolai;Bi, Shusheng;Zhu, Xiaofeng;Liu, Jinhua
光学显微镜中基于视觉传感的三维粒子定位对于基础研究和实际应用都具有重要意义。与横向(X和Y)定位相比，实现高分辨率的轴向粒子定位更具挑战性。在这项研究中，我们旨在通过解析的方法来研究不同因素对轴向测量分辨率的影响。为了模拟光学显微镜中的三维粒子成像，建立了分析模型。采用半径向量投影法将模拟的粒子图像转换为半径向量。利用得到的半径向量，提出了轴向变化率的概念来评价轴向粒子局部化的测量分辨率。还进行了实验，并与仿真结果进行比较。此外，利用该方法还讨论了颗粒尺寸对测量分辨率的影响。结果表明，该方法为研究轴向粒子局部化的分辨率提供了一种有效的途径。
Visual sensing based three dimensional (3D) particle localization in an optical microscope is important for both fundamental studies and practical applications. Compared with the lateral (X and Y) localization, it is more challenging to achieve a high resolution measurement of axial particle location. In this study, we aim to investigate the effect of different factors on axial measurement resolution through an analytical approach. Analytical models were developed to simulate 3D particle imaging in an optical microscope. A radius vector projection method was applied to convert the simulated particle images into radius vectors. With the obtained radius vectors, a term of axial changing rate was proposed to evaluate the measurement resolution of axial particle localization. Experiments were also conducted for comparison with that obtained through simulation. Moreover, with the proposed method, the effects of particle size on measurement resolution were discussed. The results show that the method provides an efficient approach to investigate the resolution of axial particle localization.
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影响因子：
4
作者：
C. M. Anderson;G. Georgiou;I. Morrison;G. V. Stevenson;R. Cherry
通讯作者：
C. M. Anderson;G. Georgiou;I. Morrison;G. V. Stevenson;R. Cherry
影响因子：
4.6
作者：
Afik E
通讯作者：
Afik E
DOI：
10.1364/josaa.26.001484
发表时间：
2009-06
期刊：
Journal of the Optical Society of America. A, Optics, image science, and vision
影响因子：
--
作者：
Zhipeng Zhang;C. Menq
通讯作者：
Zhipeng Zhang;C. Menq
DOI：
10.1073/pnas.0900245106
发表时间：
2009-03-03
影响因子：
11.1
作者：
Pavani, Sri Rama Prasanna;Thompson, Michael A.;Moerner, W. E.
通讯作者：
Moerner, W. E.
影响因子：
3.4
作者：
GHOSH, RN;WEBB, WW
通讯作者：
WEBB, WW