Interplay Between miR-155, AT1R A1166C Polymorphism, and AT1R Expression in Young Untreated Hypertensives
Interplay Between miR-155, AT1R A1166C Polymorphism, and AT1R Expression in Young Untreated Hypertensives
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DOI：
10.1038/ajh.2010.211
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发表时间：
2011-02-01
影响因子：
3.2
通讯作者：
Semplicini, Andrea
中科院分区：
文献类型：
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作者：
Ceolotto, Giulio;Papparella, Italia;Semplicini, Andrea
位于人血管紧张素II 1型受体（AT 1 R）3 '-UTR（非翻译区）的沉默多态性（+1166 A/C单核苷酸多态性）与高血压和心血管并发症相关。+1166 A/C被特定的微小RNA-155（miR-155）识别，该微小RNA-155与+1166 A-等位基因碱基配对互补，但与突变的+1166 C等位基因不互补。本研究的目的是探讨miR-155和AT 1 R蛋白表达之间的相互作用。方法从高血压和动态记录韦内蒂亚研究（HARVEST）的高血压患者队列（n = 573）中选择64名受试者进行+1166 A/C：1166 A等位基因纯合型25例，杂合型20例，1166 C等位基因纯合型19例。CC组AT 1 R蛋白表达明显高于AA和AC组（P
BACKGROUNDA silent polymorphism (+1166 A/C single-nucleotide polymorphism) localized in the 3'-UTR (untranslated region) of the human angiotensin II type-1 receptor (AT1R) has been associated with hypertension and cardiovascular complications. The +1166 A/C is recognized by a specific microRNA-155 (miR-155), which is base-pairing complementary with the +1166 A-allele but not with the mutant +1166 C allele. Aim of our study was to investigate the interplay between miR-155 and AT1R protein expression.METHODSSixty-four subjects were selected for the +1166 A/C from the cohort of hypertensives (n = 573) of the Hypertension and Ambulatory Recording Venetia Study (HARVEST): 25 were homozygous for the 1166 A allele, 20 heterozygous, and 19 homozygous for the 1166C allele.RESULTSmiR-155 expression was significantly decreased in subjects with CC genotype in comparison to AA and AC genotype. AT1R protein expression was significantly increased in the CC group in comparison to AA and AC (P