Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries
Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries
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在 100 个毛细管的多重阵列中同时监测通过电泳分离的 DNA 片段
DOI：
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
E. Yeung
中科院分区：
文献类型：
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作者：
K. Ueno;E. Yeung
各种激励计划分配激光束的阵列中的大量毛细管进行评估。这导致了一个多路复用系统的建设，用于监测电泳的DNA片段在100个毛细管。来自每个毛细管的激光激发的荧光信号由CCD相机以0.6帧/秒的速率同时记录。重建的电泳图显示出良好的重现性和最小的串扰。该系统提供了两个同时激发波长，使得它可以适用于基于商业四染料化学的双色、双强度DNA测序。每条激光线仅使用20 mW。进一步发展这个系统，以适应高达4096个独立的测序通道的时间进行了讨论。参考文献26篇，图6，2个标签。
Various excitation schemes for distributing a laser beam to a large number of capillaries in an array are evaluated. This led to the construction of a multiplexed system for monitoring the electrophoresis of DNA fragments in 100 capillaries. The laser-excited fluorescence signals from each capillary are simultaneously recorded at the rate of 0.6 frame/s by a CCD camera. The reconstructed electropherograms show excellent reproducibility and minimal cross-talk. The system provides for two simultaneous excitation wavelengths so that it can be adapted for two-color, two-intensity DNA sequencing based on the commercial four-dye chemistry. Only 20 mW per laser line was employed. Further development of this system to accommodate up to 4096 independent sequencing channels at a time is discussed. 26 refs., 6 figs., 2 tabs.
使用毛细管阵列电泳高速平行分离 DNA 限制性片段。
影响因子：
2.9
作者：
Clark,SM;Mathies,RA
通讯作者：
Mathies,RA