An integrated, multiparametric flow cytometry chip using "microfluidic drifting" based three-dimensional hydrodynamic focusing
An integrated, multiparametric flow cytometry chip using "microfluidic drifting" based three-dimensional hydrodynamic focusing
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DOI：
10.1063/1.3701566
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发表时间：
2012-06-01
期刊：
影响因子：
3.2
通讯作者：
Huang, Tony Jun
中科院分区：
文献类型：
--
作者：
Mao, Xiaole;Nawaz, Ahmad Ahsan;Huang, Tony Jun
在这项工作中，我们展示了一个集成的，单层的，微型流式细胞仪设备，能够进行多参数颗粒分析。该装置在芯片上集成了颗粒聚焦和检测组件，包括基于“微流体漂移”的三维（3D）流体动力学聚焦组件和集成到微流体架构中以促进芯片上检测的一系列光纤。利用这种设计，多个光信号（即，前向散射、侧向散射和荧光）。实验结果表明，我们的流式细胞仪芯片的性能是其庞大的，昂贵的桌面对应。将片上3D颗粒聚焦与片上多参数光学检测集成在单层、可大规模生产的微流体装置中，向用于即时临床诊断的低成本流式细胞术芯片迈出了重要一步。(C)2012年美国物理学会。[http：//dx.doi.org/10.1063/1.3701566]
In this work, we demonstrate an integrated, single-layer, miniature flow cytometry device that is capable of multi-parametric particle analysis. The device integrates both particle focusing and detection components on-chip, including a "microfluidic drifting" based three-dimensional (3D) hydrodynamic focusing component and a series of optical fibers integrated into the microfluidic architecture to facilitate on-chip detection. With this design, multiple optical signals (i.e., forward scatter, side scatter, and fluorescence) from individual particles can be simultaneously detected. Experimental results indicate that the performance of our flow cytometry chip is comparable to its bulky, expensive desktop counterpart. The integration of on-chip 3D particle focusing with on-chip multi-parametric optical detection in a single-layer, mass-producible microfluidic device presents a major step towards low-cost flow cytometry chips for point-of-care clinical diagnostics. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.3701566]