A bio-sniffer stick with FALDH (formaldehyde dehydrogenase) for convenient analysis of gaseous formaldehyde
A bio-sniffer stick with FALDH (formaldehyde dehydrogenase) for convenient analysis of gaseous formaldehyde
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DOI：
10.1016/j.snb.2007.07.086
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发表时间：
2008-03-14
影响因子：
8.4
通讯作者：
Marty, Jean-Louis
中科院分区：
文献类型：
--
作者：
Mitsubayashi, Kohji;Nishio, Genki;Marty, Jean-Louis
研制了一种具有甲醛脱氢酶活性的棒状生物化学气体传感器（生物嗅探器），该传感器具有气体选择性好、检测方便的特点。将酶固定在亲水性聚四氟乙烯膜上，构建了一种用于检测气相中甲醛的生物嗅探器。采用安培法测定了NADH酶促反应产生的NADH氧化电流，并对气相中甲醛进行了标定，标定范围为40 ~ 3000 ppb，满足了住宅中甲醛蒸气的最大允许浓度（80 ppb）和人体嗅觉甲醛检测限（410 ppb）。基于BMDH作为气体识别材料的底物特异性，BMDH固定化嗅探器表现出良好的气体选择性，作为环境应用的结果，BMDH固定化嗅探器可用于建筑木材作为外部框架材料中甲醛浓度的测定。通过回归分析计算的浓度值与市售气体传感器和甲醛气体检测管的浓度值一致。(C)2007 Elsevier B. V.保留所有权利。
A stick type biochemical gas sensor (bio-sniffer) with formaldehyde dehydrogenase (FALDH) was developed for convenient analysis of gaseous formaldehyde with high gas selectivity. The bio-sniffer for formaldehyde in the gas phase was constructed by immobilizing enzyme to a Pt electrode-coated hydrophilic PTFE membrane. The oxidation current of NADH produced by the FALDH enzymatic reactions was measured by amperometric analysis.The calibration ranges of the bio-sniffer for formaldehyde in the gas phase was from 40 to 3000 ppb, which encountered the maximum permissible concentration of formaldehyde vapor in the residential house (80 ppb) and formaldehyde detection limit for the human sense of smell (410 ppb). The FALDH immobilized sniffer indicated good gas selectivity based on the substrate specificity of FALDH as gas recognition material.As the results of the environmental application, the bio-sniffer was possible to measure the formaldehyde concentration from a building timber as an exterior frame material. The calculated concentration value by a regression analysis was consistent with those of a commercially available gas sensor and a gas detector tube for formaldehyde. (C) 2007 Elsevier B.V. All rights reserved.