Modelling the helium plasma jet delivery of reactive species into a 3D cancer tumour
Modelling the helium plasma jet delivery of reactive species into a 3D cancer tumour
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DOI：
10.1088/1361-6595/aa9b3b
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发表时间：
2018-01-01
影响因子：
3.8
通讯作者：
Short, Robert D.
中科院分区：
文献类型：
--
作者：
Szili, Endre J.;Oh, Jun-Seok;Short, Robert D.
低温大气等离子体因其在癌症治疗中的潜在有益作用而引起了全世界的广泛关注。为了进一步提高血浆在癌症治疗中的有效性，重要的是要了解血浆反应物质的产生和转运到组织液、组织和细胞中，以及输送的速度和深度，特别是跨越皮肤等物理屏障。在这项研究中，氦（He）等离子体喷射治疗生长在活体小鼠背部的三维癌症肿瘤，诱导肿瘤内2.8 mm深度的细胞凋亡。氦等离子体射流在穿透肿瘤的整个深度之前，可以通过完整的皮肤屏障输送活性氧。然后在琼脂糖组织模型中跟踪He等离子体射流产生H2O2， NO3-和NO2-以及水氧[O-2(aq)]的深度和传输速率。这提供了H2O2、NO3-、NO2-和O-2（aq）浓度的近似值，这些浓度可能是在He等离子体喷射治疗3D肿瘤期间产生的。有人提出He等离子体射流可以通过H2O2、NO3和NO2与O-2（aq）的“深度”传递诱导肿瘤内的细胞凋亡；后者提高缺氧组织的氧张力。
Cold atmospheric plasmas have attracted significant worldwide attention for their potential beneficial effects in cancer therapy. In order to further improve the effectiveness of plasma in cancer therapy, it is important to understand the generation and transport of plasma reactive species into tissue fluids, tissues and cells, and moreover the rates and depths of delivery, particularly across physical barriers such as skin. In this study, helium (He) plasma jet treatment of a 3D cancer tumour, grown on the back of a live mouse, induced apoptosis within the tumour to a depth of 2.8 mm. The He plasma jet was shown to deliver reactive oxygen species through the unbroken skin barrier before penetrating through the entire depth of the tumour. The depth and rate of transport of He plasma jet generated H2O2, NO3- and NO2-, as well as aqueous oxygen [O-2(aq)], was then tracked in an agarose tissue model. This provided an approximation of the H2O2, NO3-, NO2- and O-2(aq) concentrations that might have been generated during the He plasma jet treatment of the 3D tumour. It is proposed that the He plasma jet can induce apoptosis within a tumour by the 'deep' delivery of H2O2, NO3 and NO2 coupled with O-2(aq); the latter raising oxygen tension in hypoxic tissue.