Gap junctions on GABAergic neurons containing the calcium-binding protein parvalbumin in the rat hippocampus (CA1 region)
Gap junctions on GABAergic neurons containing the calcium-binding protein parvalbumin in the rat hippocampus (CA1 region)
复制标题
大鼠海马（CA1 区）含有钙结合蛋白小清蛋白的 GABA 能神经元上的间隙连接
作者：
H. Katsumaru;Toshio Kosaka;Claus W. Heizmann;K. Hama
首次在中枢神经系统中化学定义的神经元上发现缝隙连接。因此，在大鼠海马内含有钙结合蛋白小白蛋白(PV)的GABA能神经元上显示了缝隙连接。薄切片和半薄切片(0.5μm厚)被交替和连续地从包埋在环氧树脂中的用于常规电子显微镜研究的Os固定组织中切割出来。用抗PV血清对半薄切片进行包埋后免疫细胞化学处理。半薄切片上的PV免疫反应(PV-I)轮廓对应的结构很容易从相邻薄切片的电子显微镜上辨认出来。利用这一技术发现了(1)PV-I树突之间的缝隙连接，(2)PV-I树突与PV-I胞体之间的缝隙连接，(3)PV-I树突与来源不明的小突起之间的缝隙连接。尽管进行了系统的搜索，但我们没有发现PV阴性过程之间的缝隙连接。
SummaryGap junctions were identified for the first time on chemically defined neurons in the central nervous system. Gap junctions were thus demonstrated on GABAergic neurons containing the calcium-binding protein parvalbumin (PV) in the rat hippocampus. Thin and semithin (0.5 μm thick) sections were cut alternately and consecutively from osmium-fixed tissue which was embedded in epoxy resin and usable for conventional electron microscopic studies. The semithin sections were processed for postembedding immunocytochemistry using an anti-PV serum. Structures corresponding to the PV-immunoreactive (PV-I) profiles on the semithin sections were easily identified on electron micrographs from the adjacent thin sections. Using this technique gap junctions were found (1) between PV-I dendrites, (2) between PV-I dendrites and PV-I somata and (3) between PV-I dendrites and small processes whose origin could not be identified. Despite a systematic search, we did not find gap junction between PV-negative processes.