MICE LACKING MHC CLASS-II MOLECULES
MICE LACKING MHC CLASS-II MOLECULES
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DOI：
10.1016/0092-8674(91)90448-8
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发表时间：
1991-09-06
期刊：
影响因子：
64.5
通讯作者：
MATHIS, D
中科院分区：
文献类型：
--
作者：
COSGROVE, D;GRAY, D;MATHIS, D
我们已经产生了缺乏主要组织相容性复合体II类抗原的小鼠，使我们能够评估这些分子在T和B细胞分化的各个方面的作用。突变小鼠显示脾脏和淋巴结的CD4+ T淋巴细胞几乎完全消除；少数剩余的cd4阳性细胞优先定位于B细胞滤泡。令人惊讶的是，大量CD4单阳性细胞存在于胸腺中；然而，这些不是我们目前所认识的成熟胸腺细胞。B淋巴细胞数量正常，能够向浆细胞终末分化。然而，B细胞区室的一些畸变是可以证明的：生发中心的缺乏，某些个体中IgM+IgD+细胞的减少，血清IgG1的产生减少，以及对t依赖性抗原完全没有反应。总之，ii类阴性小鼠证实了一些关于淋巴细胞分化的旧观念，但也提供了一些惊喜。
We have produced mice that lack major histocompatibility complex class II antigens, permitting us to evaluate the role of these molecules in diverse aspects of T and B cell differentiation. The mutant mice show near-complete elimination of CD4+ T lymphocytes from the spleen and lymph nodes; the few remaining CD4-positive cells are preferentially localized to B cell follicles. Surprisingly, substantial numbers of CD4 single-positive cells reside in the thymus; however, these are not mature thymocytes as we currently recognize them. B lymphocytes occur in normal numbers and are capable of terminal differentiation to plasma cells. Nevertheless, several aberrations in the B cell compartment are demonstrable: a lack of germinal centers, fewer IgM+IgD+ cells in certain individuals, reduced production of serum IgG1, and complete inability to respond to T-dependent antigens. In short, the class II-negative mice have confirmed some old ideas about lymphocyte differentiation, but have provided some surprises.