A highly selective turn-on fluorescent probe for Al(III) based on coumarin and its application in vivo.
A highly selective turn-on fluorescent probe for Al(III) based on coumarin and its application in vivo.
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DOI：
10.1039/c3an02247a
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发表时间：
2014-03
期刊：
影响因子：
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通讯作者：
Hongde Xiao;Kun Chen;Nannan Jiang;Dandan Cui;G. Yin;Jie Wang;Ruiyong Wang
中科院分区：
文献类型：
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作者：
Hongde Xiao;Kun Chen;Nannan Jiang;Dandan Cui;G. Yin;Jie Wang;Ruiyong Wang
本研究以香豆素为荧光探针，合成了一种7-羟基-6-[（2-羟基-萘-1-基亚甲基）-氨基]-4-甲基-色满-2-酮（CN），用于检测水溶液中的Al（3+）。根据Job图和ESI-MS数据确定CN-Al（3+）络合物的结合比为1：1。由Benesi-Hildebrand图计算出Al（3+）与CN的结合常数（Ka）为9.55 × 10（4）M（-1），检出限为0.10 μM（LOD = 3σ/斜率）。CN可作为检测HeLa细胞中Al（3+）的有效荧光探针。此外，CN还可用于斑马鱼体内Al（3+）的检测。
In this study, a turn-on coumarin-based fluorescent probe, 7-hydroxy-6-[(2-hydroxy-naphthalen-1-ylmethylene)-amino]-4-methyl-chroman-2-one (CN), was developed for detecting Al(3+) in aqueous systems. The binding ratio of CN-Al(3+) complexes was determined from the Job plot and ESI-MS data to be 1 : 1. The binding constant (Ka) of Al(3+) binding to CN was calculated to be 9.55 × 10(4) M(-1) from a Benesi-Hildebrand plot and the detection limit was evaluated to be as low as 0.10 μM (LOD = 3σ/slope). CN could be used as an effective fluorescent probe for detecting Al(3+) in living HeLa cells. Moreover, CN could also be applied in the in vivo detection of Al(3+) in zebrafish.