Testing chromosomal phylogenies and inversion breakpoint reuse in Drosophila
Testing chromosomal phylogenies and inversion breakpoint reuse in Drosophila
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DOI：
10.1534/genetics.106.062612
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发表时间：
2007-01-01
期刊：
影响因子：
3.3
通讯作者：
Ruiz, Alfredo
中科院分区：
文献类型：
--
作者：
Gonzalez, Josefa;Casals, Ferran;Ruiz, Alfredo
采用细胞遗传学和生物信息学相结合的方法，研究了果蝇（Drosophila buzzatii）与果蝇（D.回复染色体X和2，窝藏这两个物种之间固定的倒位，进行了分析。首先，通过比较原位杂交，鉴定了两个物种分化过程中保守的染色体片段。repleta染色体的180个BAC克隆从BAC为基础的物理图的D。buzzath基因组。这些保守的片段精确地界定与含有反转断点的克隆的帮助。然后使用GRIMM软件估计将一个基因组转化为另一个基因组所需的最小重排次数，并确定所有可能的重排方案。最后，最合理的倒位轨迹进行了测试，通过杂交12个断点轴承BAC克隆的染色体中的epleta组的其他七个物种。结果表明，D. buzzatii和D. replela有12个臂旁倒位。其中9个固定在2号染色体上，需要两个断点重复使用。我们的研究结果还表明，D。repleta和D. mercatoruin比D. repleta和D. peninsularis，我们建议，在这个谱系的repleta组的系统发育关系重新考虑。Te还估计了A repleta和D之间的重排速率。buzzatii和得出结论，在果蝇属内的利率之间的血统，甚至在一个单一的物种组有很大的差异。
A combination of cytogenetic and bioinformatic procedures was used to test the chromosomal phylogeny relating Drosophila buzzatii with D. repleta. Chromosomes X and 2, harboring most of the inversions fixed between these two species, were analyzed. First, chromosomal segments conserved during the divergence of the two species were identified by comparative in situ hybridization to the D. repleta chromosomes of 180 BAC clones from a BAC-based physical map of the D. buzzath genome. These conserved segments were precisely delimited with the aid of clones containing inversion breakpoints. Then GRIMM software was used to estimate the minimum number of rearrangements necessary to transform one genome into the other and identify all possible rearrangement scenarios. Finally, the most plausible inversion trajectory was tested by hybridizing 12 breakpoint-bearing BAC clones to the chromosomes of seven other species in the epleta group. The results show that chromosomes X and 2 of D. buzzatii and D. replela differ by 12 paracentric inversions. Nine of them are fixed in chromosome 2 and entail two breakpoint reuses. Our results also show that the cytological relationship between D. repleta and D. mercatoruin is closer than that between D. repleta and D. peninsularis, and we propose that the phylogenetic relationships in this lineage of the repleta group be reconsidered. '"Te also estimated the rate of rearrangement between A repleta and D. buzzatii and conclude that rates within the genus Drosophila vary substantially between lineages, even within a single species group.