Calreticulin, a potential vascular regulatory protein, reduces intimal hyperplasia after arterial injury
Calreticulin, a potential vascular regulatory protein, reduces intimal hyperplasia after arterial injury
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DOI：
10.1161/01.atv.17.11.2359
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发表时间：
1997-11-01
影响因子：
8.7
通讯作者：
Lucas, A
中科院分区：
文献类型：
--
作者：
Dai, E;Stewart, M;Lucas, A
动脉损伤的血栓和炎症反应都与动脉粥样硬化斑块的生长有关。钙网蛋白是一种普遍存在的钙结合蛋白，具有抗血栓活性，此外，还与白细胞活化有关。我们正在研究钙网蛋白作为一种潜在的血管调节蛋白。对91只大鼠髂股动脉球囊损伤部位内膜增生的发生进行了研究。在球囊损伤前立即将钙网蛋白直接注入动脉，然后在随访4周时评估斑块的生长情况。对每个钙网蛋白结构域以及相关钙结合蛋白钙调蛋白的作用进行了平行研究；钙调钙素对血小板活化、凝块形成和单核细胞迁移的影响也进行了研究。当在大鼠髂股动脉球囊损伤前灌注钙网蛋白或其高容量Ca2+结合C域时，可显著减少斑块的形成，而钙钙蛋白（一种相关的钙结合蛋白，缺乏钙网蛋白的多功能性质）不会减少斑块面积(生理盐水：0.037+/-0.007 mm(2)，钙钙蛋白：0.042+/-0.021 mm(2)，钙网蛋白：0.003+/-0.002 mm(2), n=46， P
Both thrombotic and inflammatory responses to arterial injury have been implicated in atherosclerotic plaque growth. Calreticulin is a ubiquitous calcium-binding protein with antithrombotic activity and, in addition, is associated with leukocyte activation. We are investigating calreticulin as a potential vascular regulatory protein. The development of intimal hyperplasia was studied at sites of balloon injury in iliofemoral arteries from 91 rats. Calreticulin was infused directly into the artery immediately before balloon injury, and plaque growth was then assessed at 4 weeks' follow-up. Parallel studies of the effects of each calreticulin domain as well as a related calcium-binding protein, calsequestrin, were examined; The effects of calreticulin on platelet activation, clot formation, and mononuclear cell migration were also studied. When infused before balloon injury in rat iliofemoral arteries, calreticulin, or its high-capacity Ca2+-binding C domain, significantly reduces plaque development, whereas calsequestrin, a related calcium-binding protein that lacks the multifunctional nature of calreticulin, does not decrease plaque area (saline: 0.037+/-0.007 mm(2), calsequestrin: 0.042+/-0.021 mm(2), calreticulin: 0.003+/-0.002 mm(2), n=46, P