Genome Diversity of Pseudomonas aeruginosa PAO1 Laboratory Strains
Genome Diversity of Pseudomonas aeruginosa PAO1 Laboratory Strains
复制标题
DOI：
10.1128/jb.01515-09
复制
发表时间：
2010-02-01
影响因子：
3.2
通讯作者：
Tuemmler, Burkhard
中科院分区：
文献类型：
--
作者：
Klockgether, Jens;Munder, Antje;Tuemmler, Burkhard
铜绿假单胞菌PAO 1是研究这种普遍存在且代谢多样的机会致病菌的最常用菌株。菌株PAO 1是原始澳大利亚PAO分离株的衍生物，已分布到世界各地的实验室和菌株保藏中心。几十年来，出现了PAO 1亚系的不一致表型。以现有的PAO 1-UW基因组序列（以领导测序项目的华盛顿大学命名）为蓝本，通过物理作图和深度短读测序合成解析参考菌株MPAO 1和PAO 1-DSM（保存在德国微生物和细胞培养物保藏中心[DSMZ]）的基因组序列。MPAO 1是转座子插入突变体近饱和文库的来源，PAO 1-DSM在其SpeI-Dpn I限制性内切酶图谱上与原始分离物相同。与PAO 1-UW相比，MPAO 1和PAO 1-DSM的主要基因组差异是缺少大倒位，携带不同整合酶和蛋白磷酸酶或激酶的移动的12-kb原噬菌体区域的重复，大小为3至1，006 bp的缺失，以及至少39个单核苷酸取代，其中17个影响蛋白质序列。PAO 1亚系在急性小鼠气道感染模型中科普营养限制的能力和毒力不同。在稳定生长后期，PAO 1-DSM亚系的数量超过其他两个亚系。总之，铜绿假单胞菌PAO 1显示出基因型和表型的持续微进化，这危及研究的可重复性。高通量基因组重测序将解决更多的病例，并可能成为菌株收集的适当质量控制。
Pseudomonas aeruginosa PAO1 is the most commonly used strain for research on this ubiquitous and metabolically versatile opportunistic pathogen. Strain PAO1, a derivative of the original Australian PAO isolate, has been distributed worldwide to laboratories and strain collections. Over decades discordant phenotypes of PAO1 sublines have emerged. Taking the existing PAO1-UW genome sequence ( named after the University of Washington, which led the sequencing project) as a blueprint, the genome sequences of reference strains MPAO1 and PAO1-DSM (stored at the German Collection for Microorganisms and Cell Cultures [DSMZ]) were resolved by physical mapping and deep short read sequencing-by-synthesis. MPAO1 has been the source of near-saturation libraries of transposon insertion mutants, and PAO1-DSM is identical in its SpeI-DpnI restriction map with the original isolate. The major genomic differences of MPAO1 and PAO1-DSM in comparison to PAO1-UW are the lack of a large inversion, a duplication of a mobile 12-kb prophage region carrying a distinct integrase and protein phosphatases or kinases, deletions of 3 to 1,006 bp in size, and at least 39 single-nucleotide substitutions, 17 of which affect protein sequences. The PAO1 sublines differed in their ability to cope with nutrient limitation and their virulence in an acute murine airway infection model. Subline PAO1-DSM outnumbered the two other sublines in late stationary growth phase. In conclusion, P. aeruginosa PAO1 shows an ongoing microevolution of genotype and phenotype that jeopardizes the reproducibility of research. High-throughput genome resequencing will resolve more cases and could become a proper quality control for strain collections.