Versican expression during skeletal/joint morphogenesis and patterning of muscle and nerve in the embryonic mouse limb.
Versican expression during skeletal/joint morphogenesis and patterning of muscle and nerve in the embryonic mouse limb.
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DOI：
10.1002/ar.a.20151
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发表时间：
2005-02
期刊：
影响因子：
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通讯作者：
Holly Snow;L. M. Riccio;C. Mjaatvedt;S. Hoffman;A. A. Capehart-A.
中科院分区：
文献类型：
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作者：
Holly Snow;L. M. Riccio;C. Mjaatvedt;S. Hoffman;A. A. Capehart-A.
Versican是一种细胞外基质蛋白聚糖，与肢体发育有关，并在前软骨间充质凝聚中表达。然而，研究缺乏精确的空间和时间的多功能蛋白聚糖定位在骨骼发育过程中，其关系的肌肉和神经的模式在哺乳动物肢体发育。转基因小鼠系hdf（心脏缺陷），它带有一个lacZ报告构建破坏Cspg 2编码多功能蛋白聚糖，允许通过组织化学分析的hdf转基因表达的检测。通过lacZ组织化学、免疫组织化学和原位杂交，评价了Hdf转基因在整装杂合子胚胎中的表达，以及多功能蛋白聚糖相对于软骨、肌肉和神经组织在石蜡包埋的10.5-14天后野生型胚胎肢体切片中的定位。Versican定位于前软骨冷凝和新生软骨内，在软骨模型的成熟过程中，在稍后的时间点表达减少。有趣的是，多功能蛋白聚糖在发育中的滑膜关节间区中仍然高度表达，这表明多功能蛋白聚糖在关节形态发生中的潜在功能。孤立的成肌细胞，初期骨骼肌群众，和神经突不存在于发展中的肢体内的强versican表达的领域。表达versican的组织可以为未来的肢体骨骼和发育中的关节保留空间，并且可以通过阻止肌肉迁移和神经支配进入这些区域来帮助肌肉和神经的图案化。
Versican, an extracellular matrix proteoglycan, has been implicated in limb development and is expressed in precartilage mesenchymal condensations. However, studies have lacked precise spatial and temporal investigation of versican localization during skeletogenesis and its relationship to patterning of muscle and nerve during mammalian limb development. The transgenic mouse line hdf (heart defect), which bears a lacZ reporter construct disrupting Cspg2 encoding versican, allowed ready detection of hdf transgene expression through histochemical analysis. Hdf transgene expression in whole mount heterozygous embryos and localization of versican relative to cartilage, muscle, and nerve tissues in paraffin-embedded limb sections of wild-type embryos from 10.5-14 days postcoitum were evaluated by lacZ histochemistry, immunohistochemistry, and in situ hybridization. Versican was localized within precartilage condensations and nascent cartilages with expression diminishing during maturation of the cartilage model at later time points. Interestingly, versican remained highly expressed in developing synovial joint interzones, suggesting potential function for versican in joint morphogenesis. Isolated myoblasts, incipient skeletal muscle masses, and neurites were not present in areas of strong versican expression within the developing limb. Versican-expressing tissues may reserve space for the future limb skeleton and developing joints and may aid in patterning of muscle and nerve by deterring muscle migration and innervation into these regions.