Anchoring Effects of Surface Chemistry on Gold Nanorods: Modulates Autophagy.
Anchoring Effects of Surface Chemistry on Gold Nanorods: Modulates Autophagy.
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表面化学对金纳米棒的锚定效应：调节自噬。
DOI：
10.1039/c5tb00076a
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发表时间：
2015-04-28
期刊：
影响因子：
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通讯作者：
Liang XJ
中科院分区：
文献类型：
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作者：
Li S;Zhang C;Cao W;Ma B;Ma X;Jin S;Zhang J;Wang PC;Li F;Liang XJ
金纳米棒（Au NRs）因其独特的生物医学特性而受到广泛关注。Au纳米核受体可以诱导纳米毒性，但这种麻烦可以通过调节自噬转化为治疗潜力。然而，Au NRs的自噬诱导活性和机制尚不清楚。我们发现表面化学修饰可以调节Au NRs在人肺腺癌A549细胞中的自噬诱导活性。CTAB包被的Au NR诱导显著水平的自噬活性，如LC 3-II转化和p62降解所证明的，而PSS和PDDAC包被的Au NR几乎不诱导自噬。更重要的是，我们还证明了AKT-mTOR信号通路负责CTAB包被的Au NR诱导的自噬。我们还发现，CTAB包被的Au NR也以时间依赖性方式诱导人胎肺成纤维细胞MRC-5细胞中的自噬。这项研究揭示了Au NRs在自噬诱导中的一个先前未知的功能，并为设计用于生物医学应用的Au NRs的表面修饰提供了新的见解。
Gold nanorods (Au NRs) have been receiving extensive attention owing to their extremely attractive properties which make them suitable for various biomedical applications. Au NRs could induce nano-toxicity, but this trouble could turn into therapeutic potential through tuning the autophagy. However, the autophagy-inducing activity and mechanism of Au NRs is still unclear. Here we showed that surface chemical modification can tune the autophagy-inducing activity of Au NRs in human lung adenocarcinoma A549 cells. CTAB-coated Au NRs induce remarkable levels of autophagy activity as evidenced by LC3-II conversion and p62 degradation, while PSS- and PDDAC-coated Au NRs barely induce autophagy. More importantly, we also demonstrated that the AKT-mTOR signaling pathway was responsible for CTAB-coated Au NRs-induced autophagy. We furthermore showed that CTAB-coated Au NRs also induces autophagy in human fetal lung fibroblast MRC-5 cells in a time-dependent manner. This study unveils a previously unknown function for Au NRs in autophagy induction, and provides a new insight for designing surface modifications of Au NRs for biomedical applications.